This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-CAG-Sec/SPIII-CD79α-CD28-Flag) and CRISPR-Cas (Cas12b (C2c1)-RNA-2xHEPN-NNNNGATT-attP) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | 1UL13 |
Host | Mouse |
Targeting Cell Type | Ramos.2G6.4C10 |
Reporter gene | YFP |
Promoter | CAG |
Signal peptides | Sec/SPIII |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | Flag |
Linkers | (SSSSG)x2 serine glycine linker |
Co-Receptors | Siglec-G |
Vector Type | Lentivirus |
Vector Name | BGpCDH2 |
Class | 2 single-Cas |
Type | II |
Subtype | A, B, C, D, E |
Effector | Cas12b (C2c1) |
DNA Type | RNA |
Nuclease domains | 2xHEPN |
TracrRNA requirement | No |
PAM/PFS | NNNNGATT |
Insertion | attP |
Construction | YFP-CAG-CD79α-Cas12b (C2c1)-RNA-attP |
Target | RXFP3 |
Alternative Names | RUNX1; AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha; runt related transcription factor 1; RUNX family transcription factor 1 |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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