RXFP3 mAb (1UL13) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR8)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-CAG-Sec/SPIII-CD79α-CD28-Flag) and CRISPR-Cas (Cas12b (C2c1)-RNA-2xHEPN-NNNNGATT-attP) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone 1UL13
Host Mouse
Targeting Cell Type Ramos.2G6.4C10
Reporter gene YFP
Promoter CAG
Signal peptides Sec/SPIII
Signaling domains CD79α
TM domains CD28
Detection tags Flag
Linkers (SSSSG)x2 serine glycine linker
Co-Receptors Siglec-G
Vector Type Lentivirus
Vector Name BGpCDH2
Class 2 single-Cas
Type II
Subtype A, B, C, D, E
Effector Cas12b (C2c1)
DNA Type RNA
Nuclease domains 2xHEPN
TracrRNA requirement No
PAM/PFS NNNNGATT
Insertion attP
Construction YFP-CAG-CD79α-Cas12b (C2c1)-RNA-attP

Target Information

Target RXFP3
Alternative Names RUNX1; AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha; runt related transcription factor 1; RUNX family transcription factor 1

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number
! For Research Use Only | Not For Clinical Use