This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-UBC-Sec/SPIII-CD79β-CD28-GST) and CRISPR-Cas (NmCas9-dsDNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | S-1-61 |
| Host | Mouse |
| Targeting Cell Type | SU-DHL-8 |
| Reporter gene | GUS |
| Promoter | UBC |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79β |
| TM domains | CD28 |
| Detection tags | GST |
| Linkers | G4S linker |
| Co-Receptors | FcγRIIB |
| Vector Type | Lentivirus |
| Vector Name | BGpCDH2 |
| Class | 1 multi-Cas |
| Type | I |
| Subtype | A, B, C |
| Effector | NmCas9 |
| DNA Type | dsDNA |
| Nuclease domains | HD/Cas3 |
| TracrRNA requirement | No |
| PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | GUS-UBC-CD79β-NmCas9-dsDNA-Stat5b |
| Target | S100B |
| Alternative Names | S100B; NEF; S100; S100-B; S100beta; S100 calcium binding protein B |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.