This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-mPGK-Tat/SPI-CD79β-CD28-Myc) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-2xHEPN-NNNNGATT-attP) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | M7-PB-E9 |
| Host | Mouse |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | YFP |
| Promoter | mPGK |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79β |
| TM domains | CD28 |
| Detection tags | Myc |
| Linkers | Short Flexible Linker |
| Co-Receptors | Siglec-G |
| Vector Type | VSV |
| Vector Name | BGpCDH7 |
| Class | 3 double-Cas |
| Type | VI |
| Subtype | A, B, C, D |
| Effector | Cas12a (Cpf1) |
| DNA Type | dsDNA |
| Nuclease domains | 2xHEPN |
| TracrRNA requirement | No |
| PAM/PFS | NNNNGATT |
| Insertion | attP |
| Construction | YFP-mPGK-CD79β-Cas12a (Cpf1)-dsDNA-attP |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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