This product is is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-CMV-Sec/SPI-CD79α-CD28-HA) and CRISPR-Cas (Cas12b (C2c1)-RNA-RuvC, HNH-NNNNGATT-Alb) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | IS-2997 |
| Host | Mouse |
| Targeting Cell Type | SU-DHL-5 |
| Reporter gene | RFP |
| Promoter | CMV |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | HA |
| Linkers | Thioredoxin with polylinker |
| Co-Receptors | CD19 |
| Vector Type | RABV |
| Vector Name | BGpCDH8 |
| Class | 2 single-Cas |
| Type | V |
| Subtype | A, B, C, D, E, F, U |
| Effector | Cas12b (C2c1) |
| DNA Type | RNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | YES |
| PAM/PFS | NNNNGATT |
| Insertion | Alb |
| Construction | RFP-CMV-CD79α-Cas12b (C2c1)-RNA-Alb |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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