This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-EFS-Sec/SPI-CD79α-CD28-Myc) and CRISPR-Cas (NmCas9-ssRNA-RuvC, HNH-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | RU2P2M |
| Host | Rabbit |
| Targeting Cell Type | SU-DHL-5 |
| Reporter gene | GUS |
| Promoter | EFS |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | Myc |
| Linkers | G4S linker |
| Co-Receptors | CD22 |
| Vector Type | AAV |
| Vector Name | BGpCDH1 |
| Class | 3 double-Cas |
| Type | I |
| Subtype | A |
| Effector | NmCas9 |
| DNA Type | ssRNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | YES |
| PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | GUS-EFS-CD79α-NmCas9-ssRNA-Stat5b |
| Target | TK1 |
| Alternative Names | TK1; TK2; Thymidine kinase 1 |
| Gene ID | 7083(Human) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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