This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-CMV+intron-Sec/SPI-CD79α-CD28-GST) and CRISPR-Cas (Cas12c (C2c3)-dsDNA-RuvC, Nuc-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | 2H7L |
| Host | Human |
| Targeting Cell Type | BC-3 |
| Reporter gene | GFP |
| Promoter | CMV+intron |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | GST |
| Linkers | G4S linker |
| Co-Receptors | CD22 |
| Vector Type | RABV |
| Vector Name | BGpCDH8 |
| Class | 1 multi-Cas |
| Type | III |
| Subtype | A |
| Effector | Cas12c (C2c3) |
| DNA Type | dsDNA |
| Nuclease domains | RuvC, Nuc |
| TracrRNA requirement | YES |
| PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | GFP-CMV+intron-CD79α-Cas12c (C2c3)-dsDNA-Stat5b |
| Target | TNF |
| Alternative Names | Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin; TNF-Alpha; TNFSF2; TNF-A; TNFA; Tumor Necrosis Factor (TNF Superfamily; Member 2); Tumor Necrosis Factor Ligand 1F |
| Gene ID | 7124(Human); 21926(Mouse) |
| UniProt ID | P01375(Human); P06804(Mouse) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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