This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-UBC-Sec/SPII-CD79α-CD28-Myc) and CRISPR-Cas (Cas13c (C2c7)-RNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | mATK2371 |
| Host | Human |
| Targeting Cell Type | Raji-Luc2 |
| Reporter gene | GFP |
| Promoter | UBC |
| Signal peptides | Sec/SPII |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | Myc |
| Linkers | Short Flexible Linker |
| Co-Receptors | CD22 |
| Vector Type | MSCV |
| Vector Name | BGpCDH6 |
| Class | 1 multi-Cas |
| Type | III |
| Subtype | A, B, C, D |
| Effector | Cas13c (C2c7) |
| DNA Type | RNA |
| Nuclease domains | HD/Cas3 |
| TracrRNA requirement | YES |
| PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | GFP-UBC-CD79α-Cas13c (C2c7)-RNA-Stat5b |
| Target | TNF |
| Alternative Names | Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin; TNF-Alpha; TNFSF2; TNF-A; TNFA; Tumor Necrosis Factor (TNF Superfamily; Member 2); Tumor Necrosis Factor Ligand 1F |
| Gene ID | 7124(Human); 21926(Mouse) |
| UniProt ID | P01375(Human); P06804(Mouse) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.