The product is a highly efficient, inducible Cas9 D10A (Nickase)-ASGR1 gene-editing based novel VACV packaging system that can be used to mediate researches involving anti-SIGLEC9 mab (5B1Q) in Murine B cell engineering.
| Clone | 5B1Q |
| Host | Humanized |
| Packaging Cell Line | CHO-K1 |
| Vector Promoter | CMV |
| Rep | Rep40 |
| Capsid | P24 |
| Envelope | VSV-G |
| Matrix | N-terminal |
| Size | ~24kb |
| Genome | ssRNA |
| Virus Type | VACV |
| Cofactor Requirement | No ATP, CP, or CPK |
| Particles/mL | 8.1 × 10¹¹ |
| PFU/mL | 7.9 × 10¹¹ |
| TU/mL | 9.2 × 10¹¹ |
| Cas9 | Cas9 D10A (Nickase) |
| sgRNA | ASGR1 |
| Engineered B Cell | Murine B cells |
| Target | SIGLEC9 |
| Alternative Names | SIGLEC9; CD329; CDw329; FOAP-9; OBBP-LIKE; siglec-9; sialic acid binding Ig like lectin 9 |
| Format | Liquid |
| Shipping | The product is shipped by dry ice. |
| Storage | Upon receipt, store immediately at -80°C or lower for long-term storage. Avoid freeze/thaw cycles. |
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