How does a multiplex assay compare to running multiple single-plex ELISAs in terms of data quality and cost?

Multiplex assays offer a major advantage by quantifying up to 50 or more analytes from the same sample volume, drastically reducing sample consumption and manual labor. While single-plex ELISAs might sometimes offer slightly higher sensitivity for one target, our validated multiplex kits provide excellent pg/mL sensitivity across all targets with better data-per-dollar value and eliminate the batch-to-batch variation inherent in running multiple independent assays.

What is the minimum sample volume required for a high-plex panel, and what sample types can be accommodated?

We design our kits to be highly efficient, typically requiring as little as 25 to 50μL of liquid sample per well for a high-plex panel. We routinely validate kits for various sample types, including serum, plasma, cell culture supernatants, tissue homogenates, and complex body fluids.

Can you develop a custom panel that includes non-cytokine targets, such as signal transduction proteins or specialized disease biomarkers?

Absolutely. Our expertise extends far beyond cytokines. We regularly develop custom multi-analyte panels that integrate targets such as intracellular signaling molecules, neurodegeneration markers, and metabolic hormones. Our platform flexibility allows us to combine these diverse targets efficiently.

We are developing a therapeutic for an exotic species. Can you develop a multiplex kit for non-traditional research models?

Yes, we are not limited to Human, Mouse, and Rat. We have successfully developed multiplex assays for a broad range of non-traditional models, including non-human primates (NHP), canine, porcine, and avian species. If validated antibodies are available, we can develop the assay; if not, we can initiate a custom antibody development project first.

Multiplex Assay Kits

Are you currently facing long drug development cycles, limited sample volumes, or the challenge of gaining a comprehensive view of complex biological pathways? Our multiplex assay kits provide a powerful solution for simultaneously quantifying multiple analytes in a single experiment, delivering comprehensive and high-quality data.

Introduction of Multiplex Assay Kit

Multiplex assays represent a paradigm shift in biological analysis, moving beyond the traditional single-target immunoassay to enable the simultaneous detection and quantification of multiple analytes. This technology, grounded in decades of biochemical and immunological research, allows for a more holistic understanding of biological systems. By consolidating multiple measurements into a single experiment, researchers can explore complex interactions and pathways that are often missed with conventional methods. This approach is highly valued in the scientific community for its efficiency and ability to generate rich, interconnected data sets that can be used for biomarker discovery.

Multiplex assay kit. (Creative Biolabs Authorized)

Streamlined Customization Process of Assay Kits

01Project Initiation & Design

Kickstart your project with a meticulously crafted Assay Design Document. This blueprint encompasses a reagent sourcing strategy, validation tactics, and a projected timeline, ensuring a seamless and efficient project launch.

02Reagent Selection & Coupling

For unparalleled assay accuracy, we meticulously source and quality-check antibodies and capture reagents for all targets. Our robust coupling process attaches these to magnetic beads or plate surfaces, yielding batch-validated reagents with optimal efficiency and minimal non-specific binding.

03Assay Optimization & Calibration

Dive deep into assay optimization, systematically testing antibody pairs, signal amplification systems, and matrix impacts. We establish mass-calibrated standards and the perfect standard curve fit, ensuring an optimized protocol with unmatched sensitivity, dynamic range, and zero cross-talk.

04Full Performance Validation

Our comprehensive testing phase includes matrix spiking, dilution linearity, and precision studies. This rigorous validation guarantees data reproducibility and robustness, culminating in a Final Validation Report detailing KPIs and reportable ranges for all analytes.

05Final Kit Manufacturing & Delivery

We finalize kit components with precision, manufacturing standards and buffers on a large scale. Accompanied by a detailed technical data sheet and certificate of analysis, our kits arrive ready to deliver exceptional performance data.

Quality Control and Reporting

Every step of the process is subjected to stringent quality control checks. The final deliverables are compiled into a comprehensive, easy-to-understand report.

Why Choose Us?

Exceptional Sensitivity

Our kits are built on a foundation of proprietary technology that delivers exceptional sensitivity and dynamic range, allowing for the detection of low-abundance proteins and a wide range of concentrations.

Streamlined Workflows

Unlike traditional methods, our streamlined workflows reduce hands-on time and minimize the risk of human error, ensuring consistent and reproducible results.

Comprehensive Data

Our multiplexing capabilities provide a full biomarker profile from a single sample, offering a more complete picture than single-analyte assays.

Customizable Solutions

We offer a broad catalog of pre-defined panels as well as the ability to develop custom panels tailored to your unique research needs.

Applications

Immunology & Inflammation Research

Uncover the intricate networks driving immune responses and inflammatory disorders with precision-engineered tools designed for high-throughput biomarker profiling.

Cardiovascular Health Research

Advance heart disease research by analyzing circulating biomarkers linked to atherosclerosis, inflammation, and cardiac dysfunction.

Toxicology & Metabolic Disorders

Streamline safety assessments and metabolic disease research with multiplex panels that reveal multi-organ toxicity and metabolic dysregulation.

Drug Discovery

Optimize preclinical pipelines and clinical outcomes with end-to-end solutions for pharmacodynamics, biomarker modulation, and treatment response tracking.

FAQs

How does a multiplex assay compare to running multiple single-plex ELISAs in terms of data quality and cost?

Multiplex assays offer a major advantage by quantifying up to 50 or more analytes from the same sample volume, drastically reducing sample consumption and manual labor. While single-plex ELISAs might sometimes offer slightly higher sensitivity for one target, our validated multiplex kits provide excellent pg/mL sensitivity across all targets with better data-per-dollar value and eliminate the batch-to-batch variation inherent in running multiple independent assays.
What is the minimum sample volume required for a high-plex panel, and what sample types can be accommodated?

We design our kits to be highly efficient, typically requiring as little as 25 to 50μL of liquid sample per well for a high-plex panel. We routinely validate kits for various sample types, including serum, plasma, cell culture supernatants, tissue homogenates, and complex body fluids.
Can you develop a custom panel that includes non-cytokine targets, such as signal transduction proteins or specialized disease biomarkers?

Absolutely. Our expertise extends far beyond cytokines. We regularly develop custom multi-analyte panels that integrate targets such as intracellular signaling molecules, neurodegeneration markers, and metabolic hormones. Our platform flexibility allows us to combine these diverse targets efficiently.
We are developing a therapeutic for an exotic species. Can you develop a multiplex kit for non-traditional research models?

Yes, we are not limited to Human, Mouse, and Rat. We have successfully developed multiplex assays for a broad range of non-traditional models, including non-human primates (NHP), canine, porcine, and avian species. If validated antibodies are available, we can develop the assay; if not, we can initiate a custom antibody development project first.

Creative Biolabs is dedicated to providing you with the most advanced solutions for your research needs. Our multiplex assay kits are a testament to our commitment to quality, innovation, and accelerating scientific discovery. Contact us today!

For Research Use Only.

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