Human ADD1 Matched Antibody Pair set for ELISA use
Capture Antibody: Rabbit purified polyclonal anti-ADD1
Detection Antibody: Mouse monoclonal anti-ADD1, IgG1 Kappa
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Antigen Detail | |
|---|---|
| Target | ADD1 |
| Description | Alpha-adducin is a protein that in humans is encoded by the ADD1 gene. Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. Polymorphism in ADD1 is associated with hypertension. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Polyclonal |
| Host Species | Rabbit |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Clone | RD10101 |
| Isotype | IgG1, kappa |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human ADD1 Matched Antibody Pair set for ELISA use Capture Antibody: Rabbit purified polyclonal anti-ADD1 Detection Antibody: Mouse monoclonal anti-ADD1, IgG1 Kappa |
| Specificity | Validated for detecting natural and recombinant human ADD1 |
| Research Area | Hypertension |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | ADD1 |
| Full Name | Adducin 1 |
| Background | Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017] |
| Alternative Names | ADD1; ADDA; adducin 1 |
| Gene ID | 118 |
| Uniprot ID | P35611 |
| GeneCards | ADD1 |
| HGNC | 243 |
| Ensembl | ENSG00000087274 |
| OMIM | 102680 |
| Involvement in Disease | Diseases associated with ADD1 include Hypertension, Essential and Actinomycosis. |
| Function | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. |
| Related Pathways | Its related pathways are Activation of cAMP-Dependent PKA and Apoptotic execution phase. |
For Research Use Only.