Human ATF1 Matched Antibody Pair set for ELISA use
Capture Antibody: Rabbit purified polyclonal anti-ATF1
Detection Antibody: Mouse monoclonal anti-ATF1, IgG1 Kappa
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Antigen Detail | |
|---|---|
| Target | ATF1 |
| Description | Cyclic AMP-dependent transcription factor ATF-1 is a protein that in humans is encoded by the ATF1 gene. This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Polyclonal |
| Host Species | Rabbit |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Clone | RD10444 |
| Isotype | IgG1, kappa |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human ATF1 Matched Antibody Pair set for ELISA use Capture Antibody: Rabbit purified polyclonal anti-ATF1 Detection Antibody: Mouse monoclonal anti-ATF1, IgG1 Kappa |
| Specificity | Validated for detecting natural and recombinant human ATF1 |
| Research Area | Transcription; Domain Families |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | ATF1 |
| Full Name | Activating Transcription Factor 1 |
| Background | This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010] |
| Alternative Names | Cyclic AMP-Dependent Transcription Factor ATF-1 |
| Gene ID | 466 |
| Uniprot ID | P18846 |
| GeneCards | ATF1 |
| HGNC | 783 |
| Ensembl | ENSG00000123268 |
| OMIM | 123803 |
| Involvement in Disease | Diseases associated with ATF1 include Melanoma Of Soft Tissue and Fibrous Histiocytoma. |
| Function | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation. |
| Related Pathways | Its related pathways are Transcriptional misregulation in cancer and Interleukin-11 Signaling Pathway. |
For Research Use Only.