Human CBFA2T2 Matched Antibody Pair set for ELISA use
Capture Antibody: Mouse monoclonal anti-CBFA2T2, IgG2a Kappa
Detection Antibody: Mouse monoclonal anti-CBFA2T2, IgG2a Kappa
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Antigen Detail | |
|---|---|
| Target | CBFA2T2 |
| Description | Protein CBFA2T2 is a protein that in humans is encoded by the CBFA2T2 gene. The protein binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV01490 |
| Isotype | IgG2a, kappa |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Biotin |
| Clone | RD103 |
| Isotype | IgG2a, kappa |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human CBFA2T2 Matched Antibody Pair set for ELISA use Capture Antibody: Mouse monoclonal anti-CBFA2T2, IgG2a Kappa Detection Antibody: Mouse monoclonal anti-CBFA2T2, IgG2a Kappa |
| Specificity | Validated for detecting natural and recombinant human CBFA2T2 |
| Research Area | Transcription |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | CBFA2T2 |
| Full Name | CBFA2/RUNX1 Partner Transcriptional Co-Repressor 2 |
| Background | In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. |
| Alternative Names | Myeloid Translocation-Related Protein 1; MTG8-Related Protein 1; ETO Homologous On Chromosome 20; Protein CBFA2T2 |
| Gene ID | 9139 |
| Uniprot ID | O43439 |
| GeneCards | CBFA2T2 |
| HGNC | 1536 |
| Ensembl | ENSG00000078699 |
| OMIM | 603672 |
| Involvement in Disease | Diseases associated with CBFA2T2 include Myeloid Leukemia and Leukemia. |
| Function | Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency (PubMed:27281218). Involved in primordial germ cell (PCG) formation. Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent manner (By similarity). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 (AML1-MTG8/ETO fusion protein) which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (By similarity). Required for the maintenance of the secretory cell lineage in the small intestine. Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (By similarity). |
For Research Use Only.