Human CCNB1 Matched Antibody Pair set for ELISA use
Capture Antibody: Mouse monoclonal anti-CCL2
Detection Antibody: Mouse monoclonal anti-CCL2, IgG2a Kappa
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Antigen Detail | |
|---|---|
| Target | CCL2 |
| Description | The chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemoattractant protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV01175 |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Biotin |
| Clone | RD10241 |
| Isotype | IgG2a, kappa |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human CCNB1 Matched Antibody Pair set for ELISA use Capture Antibody: Mouse monoclonal anti-CCL2 Detection Antibody: Mouse monoclonal anti-CCL2, IgG2a Kappa |
| Specificity | Validated for detecting natural and recombinant human CCNB1 |
| Research Area | Immunology |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | CCL2 |
| Full Name | Chemokine (C-C motif) ligand 2 |
| Background | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. |
| Alternative Names | HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30 |
| Gene ID | 6347 |
| Uniprot ID | P13500 |
| GeneCards | CCL2 |
| HGNC | 10618 |
| Ensembl | ENSG00000108691 |
| OMIM | 158105 |
| Involvement in Disease | Diseases associated with CCL2 include Neural Tube Defects and Human Immunodeficiency Virus Type 1. |
| Function | Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:9837883, PubMed:10587439, PubMed:10529171). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:9837883, PubMed:10587439). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8627182, PubMed:9792674, PubMed:8195247). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690). |
| Related Pathways | Its related pathways are G-protein signaling Ras family GTPases in kinase cascades (scheme) and Folate Metabolism. |
For Research Use Only.