Human CD5L Matched Antibody Pair set for ELISA useCapture Antibody: Mouse monoclonal anti-CD5LDetection Antibody: Rabbit purified polyclonal anti-CD5L
Specific Inquiry(Note: Please select the requirements to fit you project. If you have any other requirements, please specify. If no specific requirements, you don't need to select any button.)
Antigen Detail | |
|---|---|
| Target | CD5L |
| Description | CD5 antigen-like is a protein that in humans is encoded by the CD5L gene. It is is a secreted glycoprotein that controls key mechanisms in inflammatory responses, with involvement in processes such as infection, atherosclerosis, and cancer. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV01909 |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Polyclonal |
| Host Species | Rabbit |
| Conjugation | Unconjugated |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human CD5L Matched Antibody Pair set for ELISA use Capture Antibody: Mouse monoclonal anti-CD5L Detection Antibody: Rabbit purified polyclonal anti-CD5L |
| Specificity | Validated for detecting natural and recombinant human CD5L |
| Research Area | Immunology |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | CD5L |
| Full Name | CD5 Molecule Like |
| Background | CD5L is an emerging key component among the repertoire of immune effectors. It was identified in 1997 as a macrophage-secreted protein, hence its original name, Spα |
| Alternative Names | CD5 Antigen-Like (Scavenger Receptor Cysteine Rich Family) |
| Gene ID | 922 |
| Uniprot ID | O43866 |
| GeneCards | CD5L |
| HGNC | 1690 |
| Ensembl | ENSG00000073754 |
| OMIM | 602592 |
| Involvement in Disease | Diseases associated with CD5L include Hereditary Elliptocytosis and Retinitis Pigmentosa 40. |
| Function | Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies (By similarity). Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed:24295828). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (PubMed:16030018, PubMed:24223991, PubMed:24583716, PubMed:25713983). |
For Research Use Only.
Related products
