Human DDIT4 Matched Antibody Pair set for ELISA use
Capture Antibody: Rabbit purified polyclonal anti-DDIT4
Detection Antibody: Mouse polyclonal anti-DDIT4
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Antigen Detail | |
|---|---|
| Target | DDIT4 |
| Description | DNA-damage-inducible transcript 4 (DDIT4) protein also known as protein regulated in development and DNA damage response 1 (REDD1) is a protein that in humans is encoded by the DDIT4 gene. DDIT4 is a mammalian target of rapamycin inhibitor and is induced by various cellular stresses; however, its critical role in GC remains poorly understood. The present study aimed to investigate the potential relationship and the underlying mechanism between DDIT4 and GC development. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Polyclonal |
| Host Species | Rabbit |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Polyclonal |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human DDIT4 Matched Antibody Pair set for ELISA use Capture Antibody: Rabbit purified polyclonal anti-DDIT4 Detection Antibody: Mouse polyclonal anti-DDIT4 |
| Specificity | Validated for detecting natural and recombinant human DDIT4 |
| Research Area | Hypoxia |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | DDIT4 |
| Full Name | DNA Damage Inducible Transcript 4 |
| Alternative Names | DNA-Damage-Inducible Transcript 4; Protein Regulated In Development And DNA Damage Response 1 |
| Gene ID | 54541 |
| Uniprot ID | Q9NX09 |
| GeneCards | DDIT4 |
| HGNC | 24944 |
| Ensembl | ENSG00000168209 |
| OMIM | 607729 |
| Involvement in Disease | Diseases associated with DDIT4 include Bladder Urothelial Carcinoma and Tuberous Sclerosis 1. |
| Function | Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death. |
| Related Pathways | Its related pathways are mTOR signaling pathway (Pathway Interaction Database) and Gene Expression. |
For Research Use Only.