Human E4F1 Matched Antibody Pair set for ELISA useCapture Antibody: Rabbit purified polyclonal anti-COMTDetection Antibody: Mouse monoclonal anti-E4F1, IgG2a Kappa
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Antigen Detail | |
|---|---|
| Target | E4F1 |
| Description | A cellular transcription factor E4F1 interacts with an E1a-inducible enhancer and mediates constitutive enhancer function in vitro. Mouse embryos lacking E4F die at an early developmental stage, whereas enforced expression of E4F1 in various cell lines inhibits cell cycle progression. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Polyclonal |
| Host Species | Rabbit |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Clone | RD10329 |
| Isotype | IgG2a, kappa |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human E4F1 Matched Antibody Pair set for ELISA use Capture Antibody: Rabbit purified polyclonal anti-COMT Detection Antibody: Mouse monoclonal anti-E4F1, IgG2a Kappa |
| Specificity | Validated for detecting natural and recombinant human E4F1 |
| Research Area | Transcription |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | E4F1 |
| Full Name | E4F Transcription Factor 1 |
| Background | The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. Alternative splicing results in multiple transcripts encoding different proteins. |
| Alternative Names | Putative E3 Ubiquitin-Protein Ligase E4F1 |
| Gene ID | 1877 |
| Uniprot ID | Q66K89 |
| GeneCards | E4F1 |
| HGNC | 3121 |
| Ensembl | ENSG00000167967 |
| OMIM | 603022 |
| Involvement in Disease | Diseases associated with E4F1 include Hemochromatosis, Type 1. |
| Function | May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription. |
| Related Pathways | Its related pathways are p53 pathway. |
For Research Use Only.
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