Human EpCAM antibody pair set for ELISA useCapture Antibody: mouse anti-EpCAM monoclonal antibodyDetection Antibody: rabbit anti-EpCAM monoclonal antibody conjugated to HRP
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Antigen Detail | |
|---|---|
| Target | EpCAM |
| Description | Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV0185 |
| Purification | Affinity Chromatography |
| Buffer | Prior to lyophilization: PBS, pH 7.4 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rabbit |
| Conjugation | HRP |
| Clone | RD10486 |
| Purification | Affinity Chromatography |
| Buffer | Prior to lyophilization: PBS, containing 50 % HRP-Protector |
General Product Property | |
|---|---|
| Product Overview | Human EpCAM antibody pair set for ELISA use Capture Antibody: mouse anti-EpCAM monoclonal antibody Detection Antibody: rabbit anti-EpCAM monoclonal antibody conjugated to HRP |
| Specificity | Quantitative determination of Human EpCAM |
| Range | 15.625-1000 pg/mL |
| Research Area | Cell Markers |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | EpCAM |
| Full Name | Epithelial cell adhesion molecule |
| Background | Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia.[5] EpCAM is also involved in cell signaling,[6] migration,[7] proliferation, and differentiation.[8] Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E.[9] Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. |
| Alternative Names | EPCAM; DIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1; epithelial cell adhesion molecule |
| Gene ID | 4072 |
| Uniprot ID | P16422 |
For Research Use Only.
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