Human IL-1β antibody pair set for ELISA useCapture Antibody: Mouse Monoclonal IgG1Detection Antibody: Mouse Monoclonal IgG1
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Antigen Detail | |
|---|---|
| Target | IL-1β |
| Description | Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Production | Cell culture |
| Clone | IV01152 |
| Isotype | IgG1 |
| Purification | Ion exchange chromatography |
| Buffer | Prior to lyophilization: 0.25 ml PBS containing 125 mM trehalose |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Production | Cell culture |
| Conjugation | Biotin |
| Clone | RD1047 |
| Isotype | IgG1 |
| Purification | Ion exchange chromatography |
| Buffer | Prior to lyophilization: 0.5 ml PBS, containing 1% BSA and 126 mM trehalose |
General Product Property | |
|---|---|
| Product Overview | Human IL-1β antibody pair set for ELISA use Capture Antibody: Mouse Monoclonal IgG1 Detection Antibody: Mouse Monoclonal IgG1 |
| Specificity | Validated for detecting natural and recombinant human IL-1β |
| Sensitivity | 2 pg/ml |
| Research Area | Inflammation; Immunology |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | IL1B |
| Full Name | Interleukin 1 beta |
| Background | Interleukin 1 beta (IL-1β) is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. |
| Alternative Names | Interleukin 1 Beta; IL1F2; Interleukin-1 Beta; IL-1 Beta; IL1-BETA |
| Gene ID | 3553 |
| Uniprot ID | P01584 |
| GeneCards | IL1B |
| HGNC | 5992 |
| Ensembl | ENSG00000125538 |
| OMIM | 147720 |
| Involvement in Disease | Diseases associated with IL1B include Gastric Cancer, Hereditary Diffuse and Toxic Shock Syndrome. |
| Function | Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. |
| Related Pathways | Its related pathways are Monoamine Transport and Bacterial infections in CF airways. |
For Research Use Only.
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