Name: Human IL-10 ELISA Matched Antibody Pair Set [ABP-Q-04-201]
SKU: ABP-Q-04-201
Price: 919.6 USD

Human IL-10 ELISA Matched Antibody Pair Set [ABP-Q-04-201] (CAT#: ABP-Q-04-201)

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Human IL-10 antibody pair set for ELISA useCapture Antibody: Mouse anti-IL-10 monoclonal antibodyDetection Antibody: rabbit anti-Human IL-10 polyclonal antibody conjugated to HRP

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Type	Size	Price	Quantity
Antibody Pair Set-Package 1	5 × 96 tests	$919.60
Antibody Pair Set-Package 2	15 × 96 tests	$1,919.66

Datasheet

MSDS

Certificate of Analysis Lookup

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Lot Number

Antigen Detail
Target	IL-10
Description	IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.
Species Reactivity	Human

Capture Antibody
Capture Clonality	Monoclonal
Host Species	Mouse
Clone	IV01471
Purification	Affinity Chromatography
Buffer	Prior to lyophilization: PBS, pH 7.4
Application	ELISA

Detection Antibody
Det-Clonality	Polyclonal
Host Species	Rabbit
Conjugation	HRP
Purification	Affinity Chromatography
Buffer	Prior to lyophilization: PBS, containing 50 % HRP-Protector

General Product Property
Product Overview	Human IL-10 antibody pair set for ELISA use Capture Antibody: Mouse anti-IL-10 monoclonal antibody Detection Antibody: rabbit anti-Human IL-10 polyclonal antibody conjugated to HRP
Specificity	Quantitative determination of Human IL-10
Range	14.06-900 pg/mL
Research Area	Adaptive Immunity
Application	ELISA
Application Notes	Optimal dilutions should be determined experimentally by the user.
Form	Lyophilized
Storage	Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size	1 Set
Sample Type	Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates
Usage Statement	Available for Research Use Only. Not for use in therapeutic or diagnostic applications.

Target Overview
Gene Symbol	IL-10
Full Name	IL10 Gene (Protein Coding)
Background	The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

For Research Use Only.

Human IL-10 ELISA Matched Antibody Pair Set [ABP-Q-04-201] (CAT#: ABP-Q-04-201)

Antigen Detail

Capture Antibody

Detection Antibody

General Product Property

Target Overview