Human IL-6 antibody pair set for ELISA useCapture Antibody: Mouse Monoclonal IgGDetection Antibody: Mouse MonoclonalIgG
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Antigen Detail | |
|---|---|
| Target | IL-6 |
| Description | Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV0152 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Biotin |
| Clone | RD1026 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.03% Proclin 300 |
General Product Property | |
|---|---|
| Product Overview | Human IL-6 antibody pair set for ELISA use Capture Antibody: Mouse Monoclonal IgG Detection Antibody: Mouse MonoclonalIgG |
| Specificity | Both the capture and the detection antibodies in this set react with Human IL-6. |
| Sensitivity | 15.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Research Area | Autoimmune; Infection Disease; Diabetes Metabolism; Cancer; Immunology |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | IL6 |
| Full Name | Interleukin 6 |
| Background | Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory muscle factor. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Many vascular smooth muscle cells also produce IL-6 as a proinflammatory cytokine. The role of IL-6 as an anti-inflammatory actin is mediated by its inhibitory effect on TNF-α and IL-1 and the activation of IL-1ra and IL-10. Early evidence suggests that in the context of a broader coronavirus pandemic, IL-6 can be used as an inflammatory marker of severe COVID-19 infection with a poor prognosis. |
| Alternative Names | IL6; BSF2; HGF; HSF; IFNB2; IL-6; BSF-2; CDF; IFN-beta-2; interleukin 6 |
| Gene ID | 3569(Human) |
| Uniprot ID | P05231(Human) |
| GeneCards | IL6 |
| HGNC | 6018 |
| Ensembl | ENSG00000136244 |
| OMIM | 147620 |
| Involvement in Disease | Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile. |
| Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. |
| Related Pathways | Its related pathways are Transcriptional misregulation in cancer and Folate Metabolism. |
For Research Use Only.
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