Human RANKL ELISA Matched Antibody Pair Set [ABP-Q-04-367] (CAT#: ABP-Q-04-367)

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Human RANKL antibody pair set for ELISA use
Capture Antibody: rabbit anti-Human RANKL monoclonal antibody
Detection Antibody: rabbit anti-Human RANKL monoclonal antibody conjugated to HRP

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Type Size Price Quantity  
Antibody Pair Set-Package 1 5 × 96 tests $919.60
Antibody Pair Set-Package 2 15 × 96 tests $1,919.66
Datasheet
MSDS
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Lot Number

Antigen Detail
TargetRANKL
DescriptionTumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein that belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.
Species ReactivityHuman

Capture Antibody
Capture ClonalityMonoclonal
Host SpeciesRabbit
CloneIV01296
PurificationAffinity Chromatography
BufferPrior to lyophilization: PBS, pH 7.4
ApplicationELISA

Detection Antibody
Det-ClonalityMonoclonal
Host SpeciesRabbit
ConjugationHRP
CloneRD10312
PurificationAffinity Chromatography
BufferPrior to lyophilization: PBS, containing 50 % HRP-Protector

General Product Property
Product OverviewHuman RANKL antibody pair set for ELISA use
Capture Antibody: rabbit anti-Human RANKL monoclonal antibody
Detection Antibody: rabbit anti-Human RANKL monoclonal antibody conjugated to HRP
SpecificityQuantitative determination of Human RANKL
Range234.38-15000 pg/mL
Research AreaInnate Immunity
ApplicationELISA
Application NotesOptimal dilutions should be determined experimentally by the user.
FormLyophilized
StorageAvoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size1 Set
Sample TypeSerum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates
Usage StatementAvailable for Research Use Only. Not for use in therapeutic or diagnostic applications.

Target Overview
Gene SymbolTNFSF11
Full NameReceptor activator of nuclear factor kappa-Β ligand
BackgroundReceptor activator of nuclear factor kappa-Β ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a protein that in humans is encoded by the TNFSF11 gene.
Alternative NamesTNFSF11; CD254; ODF; OPGL; OPTB2; RANKL; TRANCE; hRANKL2; sOdf; TNLG6B; tumor necrosis factor superfamily member 11; TNF superfamily member 11
Gene ID8600
Uniprot IDO14788

For Research Use Only.

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