Human TNFα antibody pair set for ELISPOT useCapture Antibody: Mouse Monoclonal IgGDetection Antibody: Mouse Monoclonal IgG
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Antigen Detail | |
|---|---|
| Target | TNFα |
| Description | Tumor necrosis factor (TNF, cachexia or cachexia protein; once called tumor necrosis factor alpha or TNFα) is a cell signaling protein (cytokine) involved in systemic inflammation, and is one of the cytokines that constitute an acute phase response. It is mainly produced by activated macrophages, although it can be produced by many other cell types, such as T helper cells, natural killer cells, neutrophils, mast cells, eosinophils, and neurons. TNF is a member of the TNF superfamily and consists of various transmembrane proteins with homologous TNF domains. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV01126 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | Preservative-free PBS, pH 7.2 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Biotin |
| Clone | RD1026 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.2, containing 0.09% sodium azide |
General Product Property | |
|---|---|
| Product Overview | Human TNFα antibody pair set for ELISPOT use Capture Antibody: Mouse Monoclonal IgG Detection Antibody: Mouse Monoclonal IgG |
| Specificity | Both the capture and the detection antibodies in this set react with human tumor necrosis factor (TNFα). |
| Research Area | Inflammation |
| Application | ELISPOT |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | TNF |
| Full Name | TNF alpha |
| Background | Tumor necrosis factor (TNF, cachexia or cachexia protein; once called tumor necrosis factor alpha or TNFα) is a cell signaling protein (cytokine) involved in systemic inflammation, and is one of the cytokines that constitute an acute phase response. It is mainly produced by activated macrophages, although it can be produced by many other cell types, such as T helper cells, natural killer cells, neutrophils, mast cells, eosinophils, and neurons. TNF is a member of the TNF superfamily and consists of various transmembrane proteins with homologous TNF domains. |
| Alternative Names | TNF; DIF; TNF-alpha; TNFA; TNFSF2; Tumour necrosis factor; TNF-α; tumor necrosis factor; TNLG1F; Tumor necrosis factor alpha |
| Gene ID | 7124(Human); 21926(Mouse) |
| Uniprot ID | P05113 (Human) |
| GeneCards | TNF-α |
| HGNC | 11892 |
| Ensembl | ENSG00000232810 |
| OMIM | 191160 |
| Involvement in Disease | Diseases associated with TNF include Asthma and Malaria. |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. |
| Related Pathways | Its related pathways are Apoptosis Modulation and Signaling and Monoamine Transport. |
For Research Use Only.
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