Human TRAIL R1 ELISA Matched Antibody Pair Set [ABP-Q-04-370] (CAT#: ABP-Q-04-370)

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Human TRAIL R1 antibody pair set for ELISA use
Capture Antibody: mouse anti-Human TRAIL R1 monoclonal antibody
Detection Antibody: rabbit anti-Human TRAIL R1 monoclonal antibody conjugated to HRP

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Type Size Price Quantity  
Antibody Pair Set-Package 1 5 × 96 tests $919.60
Antibody Pair Set-Package 2 15 × 96 tests $1,919.66
Datasheet
MSDS
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Lot Number

Antigen Detail
TargetTRAIL R1
DescriptionTumor necrosis factor receptor superfamily, member 10a (TRAIL R1), also known as TRAIL receptor 1 (TRAIL R1) or CD261 antigen, is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R1/CD261/TNFRSF10A serves as a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF10A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types.
Species ReactivityHuman

Capture Antibody
Capture ClonalityMonoclonal
Host SpeciesMouse
CloneIV0176
PurificationAffinity Chromatography
BufferPrior to lyophilization: PBS, pH 7.4
ApplicationELISA

Detection Antibody
Det-ClonalityMonoclonal
Host SpeciesRabbit
ConjugationHRP
CloneRD10407
PurificationAffinity Chromatography
BufferPrior to lyophilization: PBS, containing 50 % HRP-Protector

General Product Property
Product OverviewHuman TRAIL R1 antibody pair set for ELISA use
Capture Antibody: mouse anti-Human TRAIL R1 monoclonal antibody
Detection Antibody: rabbit anti-Human TRAIL R1 monoclonal antibody conjugated to HRP
SpecificityQuantitative determination of Human TRAIL R1
Range2.34-150 pg/mL
Research AreaApoptosis
ApplicationELISA
Application NotesOptimal dilutions should be determined experimentally by the user.
FormLyophilized
StorageAvoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size1 Set
Sample TypeSerum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates
Usage StatementAvailable for Research Use Only. Not for use in therapeutic or diagnostic applications.

Target Overview
Gene SymbolTNFRSF10A
Full NameTNF Receptor Superfamily Member 10a
BackgroundThe protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Alternative NamesCD261; TRAILR1; Death Receptor 4
Gene ID8797
Uniprot IDO00220

For Research Use Only.

Application

AgonistAgonist
AntagonistAntagonist
ApopApoptosis Assay
BlockBlocking
BIBioimaging
ChIPChromatin Immunoprecipitation
Co-IPCo-Immunoprecipitation
CytCytotoxicity Assay
CellActCell Activiation
CellDiffCell Differentiation
DBDot Blot
ECLElectrochemiluminescence
ELISAEnzyme-Linked Immunosorbent Assay
Indirect ELISAIndirect ELISA
Sandwich ELISASandwich ELISA
Competitive ELISACompetitive ELISA
ELISPOTEnzyme-linked Immunosorbent Spot
FuncSFunctional Studies
FACSFluorescence-activated cell sorting
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
GSGel Shift
HAHemagglutination Assay
HPLCHigh Performance Liquid Chromatography
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IEMImmuno-Electron Microscopy
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibitInhibition Assay
Live-cell imagingLive-cell Imaging Assay
MeDIPMethylated DNA Immunoprecipitation
MAMicroagglutination Assay
MSMass Spectrometry
NeutNeutralization Assay
PepArrPeptide Array
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
RIPRadioimmunoprecipitation
StimStimulation Assay
TCAT-Cell Activation Assay
TRFTime-Resolved Fluorescence
TCTissue Culture
TBTurbidimetry
PBAProtein Binding Assay
PLAProximity Ligation Assay
SDS-PAGESodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
SPRSurface Plasmon Resonance
WBWestern Blot
CLIAChemiluminescent Immunoassay
CLEIAChemiluminescent Enzyme Immunoassay
ECLIAElectrochemiluminescence Immunoassay
TRFIATime-resolved Fluorescence Immunoassay
LIALaser Immunoassay
MLEIAMicroparticle Luminescence Enzyme Immunoassay
LETIALatex Particle-Enhanced Turbidimetric Immunoassay
LFIALateral Flow Immunoassay
GICAColloidal Gold immunochomatography Assay
ICTImmune Chromatographic Test
LTIALatex Turbidimetric Immunoassay
CMIAChemiluminescence microparticle immuno assay
IACInnuno-Affinity Chromatography
TIATurbidimetric Immunoassay

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