Monkey IFNγ antibody pair set for ELISA useCapture Antibody: Mouse Monoclonal IgG1Detection Antibody: Mouse Monoclonal IgG1
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Antigen Detail | |
|---|---|
| Target | IFN-γ |
| Description | Interferon-gamma (IFN-γ), also known as type II interferon, is produced by T and NK cells, and in smaller amounts by dendritic cells and macrophages. IFN-γ is controlled by cytokines such as IL-12 and IL-18 secreted in response to infection |
| Species Reactivity | Marmoset |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Production | Cell culture |
| Clone | IV01284 |
| Isotype | IgG1 |
| Purification | DEAE ion exchange chromatography |
| Buffer | Prior to lyophilization: 0.25 ml PBS containing 125 mM trehalose |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Production | Cell culture |
| Conjugation | Biotin |
| Clone | RD1042 |
| Isotype | IgG1 |
| Purification | DAE ion exchange chromatography |
| Buffer | Prior to lyophilization: 0.5 ml PBS, containing 1% BSA and 125 mM trehalose |
General Product Property | |
|---|---|
| Product Overview | Monkey IFNγ antibody pair set for ELISA use Capture Antibody: Mouse Monoclonal IgG1 Detection Antibody: Mouse Monoclonal IgG1 |
| Specificity | Validated for detecting natural and recombinant marmoset IFN-γ. |
| Sensitivity | 1 pg/ml |
| Research Area | Inflammation; Immunology |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | IFN gamma |
| Full Name | Interferon gamma |
| Background | Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes. It was also shown to be produced in human lymphocytes. or tuberculin-sensitized mouse peritoneal lymphocytes challenged with PPD; the resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. Those reports also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for tuberculosis. In humans, the IFNγ protein is encoded by the IFNG gene. |
| Alternative Names | Interferon_gammaIPR002069; Immune Interferon; IFN-Gamma; IFNG; IFG; IFI |
| Gene ID | 3458 |
| Uniprot ID | P01579 |
| HGNC | 5438 |
| Ensembl | ENSG00000111537 |
| OMIM | 147570 |
| Involvement in Disease | Diseases associated with IFNG include Hepatitis C Virus and Tuberous Sclerosis 2. |
| Function | IFNγ, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. IFNγ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFNγ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNγ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops as part of the adaptive immune response. IFNγ is also produced by non-cytotoxic innate lymphoid cells (ILC), a family of immune cells first discovered in the early 2010s. |
| Related Pathways | Its related pathways are Folate Metabolism and Tuberculosis. |
For Research Use Only.
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