Monkey IL-23 antibody pair set for ELISA useCapture Antibody: Mouse Monoclonal IgG1Detection Antibody: Mouse Monoclonal IgG1
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Antigen Detail | |
|---|---|
| Target | IL-23 |
| Description | Interleukin-23 (IL-23) is a heterodimeric cytokine composed of an IL12B (IL-12p40) subunit (that is shared with IL12) and the IL23A (IL-23p19) subunit.[1] IL-23 is part of IL-12 family of cytokines.[2] A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12R β1 and IL-23R.[3] Adnectin-2 is binding to IL-23 and compete with IL-23/IL-23R.[4] mRNA of IL-23R is 2,8 kB in length and includes 12 exons. The translated protein contains 629 amino acids, which is a type I penetrating protein includes signal peptide, an N-terminal fibronectin III-like domain and an intracellular part contains 3 potential tyrosine phosphorylation domains.[5] There are 24 variants of splicing of IL-23R in mitogen-activated lymphocytes.[6] IL-23R has some single nucleotide polymorphisms in the domain of binding IL-23 so there can be differences in activation of Th17.[7] There is also variant of IL-23R which has just extracellular part and it´s known as soluble IL-23R. This form can compete with membrane form to bind IL-23 and there can be difference in activation of Th17 immune response and regulation of inflammation and immune function. |
| Species Reactivity | Monkey |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Production | Cell culture |
| Clone | IV01388 |
| Isotype | IgG1 |
| Purification | Ion exchange chromatography |
| Buffer | Prior to lyophilization: 0.25 ml PBS containing 125 mM trehalose |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Production | Cell culture |
| Conjugation | Biotin |
| Clone | RD10175 |
| Isotype | IgG1 |
| Purification | Ion exchange chromatography |
| Buffer | Prior to lyophilization: 0.5 ml PBS containing 1% BSA and 125 mM trehalose |
General Product Property | |
|---|---|
| Product Overview | Monkey IL-23 antibody pair set for ELISA use Capture Antibody: Mouse Monoclonal IgG1 Detection Antibody: Mouse Monoclonal IgG1 |
| Specificity | Validated for detecting natural rhesus macaque and cynomolgus monkey IL-23 in supernatant. |
| Sensitivity | 8 pg/mL |
| Research Area | Immunology; Inflammation |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | IL23A |
| Full Name | Interleukin-23 |
| Background | IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration into tumours. IL-23 mediates its effects on both innate and adaptive arms of the immune system that express the IL-23 receptor. Th17 cells represent the most prominent T cell subset that responds to IL-23, although IL-23 has been implicated in inhibiting the development of regulatory T cell development in the intestine. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of other proinflammatory molecules such as IL-1, IL-6, TNF-alpha, NOS-2, and chemokines resulting in inflammation. |
| Alternative Names | IL23A; IL-23; IL-23A; IL23P19; P19; SGRF; Interleukin 23; interleukin 23 subunit alpha |
| Gene ID | 51561 |
| Uniprot ID | Q9NPF7 |
| GeneCards | IL23A |
| HGNC | 15488 |
| Ensembl | ENSG00000110944 |
| OMIM | 605580 |
| Involvement in Disease | Diseases associated with IL23A include Multiple Sclerosis and Inflammatory Bowel Disease. |
| Function | Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. |
| Related Pathways | Its related pathways are Tuberculosis and PEDF Induced Signaling. |
For Research Use Only.
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