Mouse E-Cadherin antibody pair set for sandwich ELISA useCapture Antibody: Monoclonal IgGDetection Antibody: Biotin-Rabbit Monoclonal IgG
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Antigen Detail | |
|---|---|
| Target | E-Cadherin |
| Description | Cadherin-1 (not to be confused with the APC C activating protein CDH1), also known as CAM 120 80 or E-cadherin or uvomorulin, is a human protein encoded by the CDH1 gene. CDH1 has also been designated as CD324 (Differentiation Cluster 324). It is a tumor suppressor gene. E-cadherin (epithelium) is the most thoroughly studied member of the cadherin family. It consists of 5 cadherin repeats in the extracellular domain (EC1 ~ EC5), a transmembrane domain and an intracellular domain that binds to p120-catenin and β-catenin. The intracellular domain contains a highly phosphorylated region, which is essential for β-catenin binding and therefore for E-cadherin function. [Citation needed] β-catenin can also be combined with α-catenin. Alpha-catenin is involved in the regulation of actin-containing cytoskeletal filaments. In epithelial cells, E-cadherin-containing intercellular junctions are usually adjacent to actin-containing filaments in the cytoskeleton. |
| Species Reactivity | Mouse |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone | IV018 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rabbit |
| Immunogen | Recombinant Mouse E-Cadherin Protein. |
| Conjugation | Biotin |
| Clone | RD1022 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Mouse E-Cadherin antibody pair set for sandwich ELISA use Capture Antibody: Monoclonal IgG Detection Antibody: Biotin-Rabbit Monoclonal IgG |
| Specificity | Both the capture and the detection antibodies in this set react with Mouse E-Cadherin. |
| Sensitivity | 7.2 pg/mL |
| Range | 31.25 pg/mL-2000 pg/mL |
| Research Area | Signal Transduction; Cancer |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | CDH1 |
| Full Name | Epithelial (E)‑Cadherin (ECAD) |
| Background | Cadherin-1 (not to be confused with the APC C activating protein CDH1), also known as CAM 120 80 or E-cadherin or uvomorulin, is a human protein encoded by the CDH1 gene. CDH1 has also been designated as CD324 (Differentiation Cluster 324). It is a tumor suppressor gene. E-cadherin (epithelium) is the most thoroughly studied member of the cadherin family. It consists of 5 cadherin repeats in the extracellular domain (EC1 ~ EC5), a transmembrane domain and an intracellular domain that binds to p120-catenin and β-catenin. The intracellular domain contains a highly phosphorylated region, which is essential for β-catenin binding and therefore for E-cadherin function. [Citation needed] β-catenin can also be combined with α-catenin. Alpha-catenin is involved in the regulation of actin-containing cytoskeletal filaments. In epithelial cells, E-cadherin-containing intercellular junctions are usually adjacent to actin-containing filaments in the cytoskeleton. |
| Alternative Names | CDH1; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; cadherin 1; BCDS1 |
| GeneCards | CDH1 |
| HGNC | 1748 |
| Ensembl | ENSG00000039068 |
| OMIM | 192090 |
| Involvement in Disease | Diseases associated with CDH1 include Blepharocheilodontic Syndrome 1 and Gastric Cancer, Hereditary Diffuse. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. |
For Research Use Only.
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