Mouse IL-12 Matched Antibody Pair Set [ABP-P-290] (CAT#: ABP-P-290)

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The antibody pair set can be used to detect Mouse IL-12 by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant IL-12.

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Antigen Detail
TargetIL-12
DescriptionThis gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Species ReactivityMouse

Capture Antibody
Capture ClonalityMonoclonal
Host SpeciesRat
ProductionAscite
CloneIV01736
IsotypeIgG
PurificationAffinity Chromatography
BufferA sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
ApplicationELISA

Detection Antibody
Det-ClonalityMonoclonal
Host SpeciesRat
ProductionAscite
ConjugationBiotin
CloneRD10245
IsotypeIgG
PurificationAffinity Chromatography
ApplicationA sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

General Product Property
Product OverviewThe antibody pair set can be used to detect Mouse IL-12 by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant IL-12.
SpecificityBoth the capture and the detection antibodies in this set react with Murine IL-12.
Research AreaImmunology
ApplicationELISA
Application NotesOptimal dilutions should be determined experimentally by the user.
FormLiquid
StorageAvoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size1 Set
Sample TypeSerum; Plasma; Cell Culture Supernatant
Usage StatementAntibody is for research use only and not for use in diagnostic or therapeutic procedures.

Target Overview
Gene SymbolIL-12
Full Nameinterleukin 12A
BackgroundThis gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Alternative NamesCLMF; Cytotoxic lymphocyte maturation factor 35 kDa subunit; NFSK; NK cell stimulatory factor chain 1; CLMF p35; NKSF1; IL-12 subunit p35; IL-12A; Interleukin-12 subunit alpha; P35
Gene ID16159(Mouse)
Uniprot IDP43431(Mouse)

For Research Use Only.

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