Mouse IL-33 Matched Antibody Pair Set [ABP-P-667] (CAT#: ABP-P-667)

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The antibody pair set can be used to detect Mouse IL-33 by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant IL-33.

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MSDS
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Lot Number

Antigen Detail
TargetIL-33
DescriptionIL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms.
Species ReactivityMouse

Capture Antibody
Capture ClonalityPolyclonal
Host SpeciesGoat
ImmunogenMouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli.
IsotypeIgG
PurificationAffinity Chromatography
BufferPBS, pH 7.4, containing 0.1% Proclin 300
ApplicationELISA

Detection Antibody
Det-ClonalityPolyclonal
Host SpeciesGoat
ImmunogenMouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli.
ConjugationBiotin
IsotypeIgG
PurificationAffinity Chromatography
ApplicationPBS, pH 7.2, containing 1% BSA (USA origin) and 0.09% sodium azide.

General Product Property
Product OverviewThe antibody pair set can be used to detect Mouse IL-33 by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant IL-33.
Research AreaImmunology
ApplicationELISA
Application NotesOptimal dilutions should be determined experimentally by the user.
FormLiquid
StorageAvoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size1 Set
Sample TypeSerum; Plasma; Cell Culture Supernatant
Usage StatementEach lot of this antibody is quality control tested by ELISA assay. A concentration of 0.125 µg/ml of the detection antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Target Overview
Gene SymbolIL-33
BackgroundIL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms.
Alternative NamesIl-1f11; Nuclear factor from high endothelial venules (NF-HEV)
Gene ID77125(Mouse)
Uniprot IDQ8BVZ5(Mouse)

For Research Use Only.

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