Mouse IL-4 antibody pair set for ELISPOT useCapture Antibody: Rat Monoclonal IgGDetection Antibody: Rat Monoclonal IgG
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Antigen Detail | |
|---|---|
| Target | IL-4 |
| Description | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Species Reactivity | Mouse |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rat |
| Clone | IV01222 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | Preservative-free PBS, pH 7.2 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rat |
| Conjugation | Biotin |
| Clone | RD105 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.2, containing 0.09% sodium azide |
General Product Property | |
|---|---|
| Product Overview | Mouse IL-4 antibody pair set for ELISPOT use Capture Antibody: Rat Monoclonal IgG Detection Antibody: Rat Monoclonal IgG |
| Specificity | Both the capture and the detection antibodies in this set react with mouse interleukin-4 (IL-4). |
| Research Area | Inflammation |
| Application | ELISPOT |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | IL4 |
| Full Name | Interleukin 4 |
| Background | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Alternative Names | Interleukin 4 |
| Gene ID | 3565(Human) |
| Uniprot ID | P05112(Human) |
| GeneCards | IL4 |
| HGNC | 6014 |
| Ensembl | ENSG00000113520 |
| OMIM | 147780 |
| Involvement in Disease | Diseases associated with IL4 include Schistosomiasis and Vernal Conjunctivitis. |
| Function | Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (By similarity). |
| Related Pathways | Its related pathways are Immune response Fc epsilon RI pathway and PEDF Induced Signaling. |
For Research Use Only.
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