Mouse IL-5 Matched Antibody Pair Set [ABP-P-655] (CAT#: ABP-P-655)

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The antibody pair set can be used to detect Mouse IL-5 by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant IL-5.

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Lot Number

Antigen Detail
TargetIL-5
DescriptionIL-5 is a homodimeric, disulphide-linked protein produced by T-cells. The native protein can have variable molecular weight as a result of glycosylation. Monomeric mouse IL-5 is a 113 amino acid protein with a reported molecular weight of 35-37 kD for the homodimeric protein.
Species ReactivityMouse

Capture Antibody
Capture ClonalityMonoclonal
Host SpeciesRat
ImmunogenPartially-purified T cell clone supernatant
CloneIV01172
IsotypeIgG1, κ
PurificationAffinity Chromatography
BufferPBS, pH 7.4, containing 0.1% Proclin 300
ApplicationELISA

Detection Antibody
Det-ClonalityMonoclonal
Host SpeciesRat
ImmunogenPartially-purified T cell clone supernatant
ConjugationBiotin
CloneRD10349
IsotypeIgG2a, kappa
PurificationAffinity Chromatography
ApplicationPBS, pH 7.2, containing 0.09% sodium azide.

General Product Property
Product OverviewThe antibody pair set can be used to detect Mouse IL-5 by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant IL-5.
Research AreaImmunology
ApplicationELISA
Application NotesOptimal dilutions should be determined experimentally by the user.
FormLiquid
StorageAvoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size1 Set
Sample TypeSerum; Plasma; Cell Culture Supernatant
Usage StatementEach lot of this antibody is quality control tested by ELISA assay. For ELISA or ELISPOT detection, the antibody should be titrated between 0.25-1.0 µg/ml to determine optimal conditions. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Target Overview
Gene SymbolIL-5
BackgroundIL-5 is a homodimeric, disulphide-linked protein produced by T-cells. The native protein can have variable molecular weight as a result of glycosylation. Monomeric mouse IL-5 is a 113 amino acid protein with a reported molecular weight of 35-37 kD for the homodimeric protein.
Alternative NamesInterleukin-5; Eosinophil differentiation factor (EDF); Eosinophil colony stimulating factor (Eo-CSF); B cell growth factor-2 (BCGF-2); B cell differentiation factor for IgM (BCDF-m); IgA enhancing factor (IgA-EF); T-cell replacing factor-1 (TRF-1)
Gene ID16191(Mouse)
Uniprot IDP04401(Mouse)

For Research Use Only.

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