Mouse Leptin antibody pair set for sandwich ELISA use
Capture Antibody: Monoclonal IgG
Detection Antibody: Biotin-Rabbit Monoclonal IgG
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Antigen Detail | |
|---|---|
| Target | Leptin |
| Description | Leptin (from the Greek λεπτός leptos, "lean") is a hormone produced mainly by fat cells and intestinal epithelial cells in the small intestine, which can regulate energy balance by suppressing starvation, thereby reducing fat storage in fat cells. Leptin acts on cell receptors in the arcuate and medial peritoneal nucleus, as well as the hypothalamus and other parts of dopaminergic neurons in the ventral tegmental area, thereby mediating eating. Although the regulation of fat storage is considered to be the main function of leptin, it also plays a role in other physiological processes, which are caused by its many synthetic sites of fat cells and hypothalamic cells with leptin receptors. Many cell types are confirmed. |
| Species Reactivity | Mouse |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone | IV0128 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rabbit |
| Immunogen | Recombinant Mouse Leptin Protein. |
| Conjugation | Biotin |
| Clone | RD105 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Mouse Leptin antibody pair set for sandwich ELISA use Capture Antibody: Monoclonal IgG Detection Antibody: Biotin-Rabbit Monoclonal IgG |
| Specificity | Both the capture and the detection antibodies in this set react with Mouse Leptin. |
| Sensitivity | 26.46 pg/mL |
| Range | 62.5 pg/mL-4000 pg/mL |
| Research Area | Neuroscience; Signal Transduction; Metabolism |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | LEP |
| Full Name | Leptin |
| Background | Leptin (from the Greek λεπτός leptos, "lean") is a hormone produced mainly by fat cells and intestinal epithelial cells in the small intestine, which can regulate energy balance by suppressing starvation, thereby reducing fat storage in fat cells. Leptin acts on cell receptors in the arcuate and medial peritoneal nucleus, as well as the hypothalamus and other parts of dopaminergic neurons in the ventral tegmental area, thereby mediating eating. Although the regulation of fat storage is considered to be the main function of leptin, it also plays a role in other physiological processes, which are caused by its many synthetic sites of fat cells and hypothalamic cells with leptin receptors. Many cell types are confirmed. |
| Alternative Names | LEP; LEPD; OB; OBS; leptin |
| GeneCards | LEP |
| HGNC | 6553 |
| Ensembl | ENSG00000174697 |
| OMIM | 164160 |
| Involvement in Disease | Diseases associated with LEP include Leptin Deficiency Or Dysfunction and Eating Disorder. |
| Function | Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (PubMed:17344214, PubMed:15899045, PubMed:19688109). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity. |
For Research Use Only.