Mouse MCP-1 Matched Antibody Pair Set [ABP-P-671] (CAT#: ABP-P-671)

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The antibody pair set can be used to detect Mouse MCP-1 by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant MCP-1.

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Lot Number

Antigen Detail
TargetMCP-1
DescriptionMonocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes.MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2.MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2.
Species ReactivityMouse

Capture Antibody
Capture ClonalityMonoclonal
Host SpeciesHamster
ImmunogenCHO-expressed, recombinant mouse MCP-1
CloneIV01275
IsotypeIgG
PurificationAffinity Chromatography
BufferPBS, pH 7.4, containing 0.1% Proclin 300
ApplicationELISA

Detection Antibody
Det-ClonalityMonoclonal
Host SpeciesArmenian Hamster
ImmunogenCHO-expressed, recombinant mouse MCP-1
ConjugationBiotin
CloneRD10210
IsotypeIgG
PurificationAffinity Chromatography
ApplicationPBS, pH 7.2, containing 0.09% sodium azide.

General Product Property
Product OverviewThe antibody pair set can be used to detect Mouse MCP-1 by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant MCP-1.
Research AreaImmunology
ApplicationELISA
Application NotesOptimal dilutions should be determined experimentally by the user.
FormLiquid
StorageAvoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size1 Set
Sample TypeSerum; Plasma; Cell Culture Supernatant
Usage StatementEach lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 6 - 10 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Additionally, BioLegend recommends the usage of Tris-Buffered Saline (TBS), pH = 7.2 for all washes and incubations, except substrate buffer. Do not use PBS as results may vary dramatically.

Target Overview
Gene SymbolMCP-1
BackgroundMonocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes.MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2.MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2.
Alternative NamesMonocyte chemoattractant protein-1; Monocyte chemoattractant and activating factor (MCAF); JE; Small inducible cytokine A2 (SCYA2); HC-11; P6; Smooth muscle cell chemotactic factor (SMC-CF); CCL2
Gene ID20296(Mouse)
Uniprot IDn/a(Mouse)

For Research Use Only.

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