Mouse p27 Kip1 antibody pair set for sandwich ELISA useCapture Antibody: Monoclonal IgGDetection Antibody: Biotin-Rabbit Monoclonal IgG
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Antigen Detail | |
|---|---|
| Target | p27 Kip1 |
| Description | Cyclin-dependent kinase inhibitor 1B (p27Kip1) is an enzyme inhibitor that in humans is encoded by the CDKN1B gene.[5] It encodes a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. It is often referred to as a cell cycle inhibitor protein because its major function is to stop or slow down the cell division cycle. |
| Species Reactivity | Mouse |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone | IV01730 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rabbit |
| Immunogen | Recombinant Mouse p27 Kip1 Protein. |
| Conjugation | Biotin |
| Clone | RD1042 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Mouse p27 Kip1 antibody pair set for sandwich ELISA use Capture Antibody: Monoclonal IgG Detection Antibody: Biotin-Rabbit Monoclonal IgG |
| Specificity | Both the capture and the detection antibodies in this set react with Mouse p27 Kip1. |
| Sensitivity | 5.8 pg/mL |
| Range | 31.25 pg/mL-2000 pg/mL |
| Research Area | Neuroscience; Signal Transduction; Metabolism |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | CDKN1B |
| Full Name | Cyclin-dependent kinase inhibitor 1B |
| Background | Cyclin-dependent kinase inhibitor 1B (p27Kip1) is an enzyme inhibitor that in humans is encoded by the CDKN1B gene.[5] It encodes a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. It is often referred to as a cell cycle inhibitor protein because its major function is to stop or slow down the cell division cycle. |
| Alternative Names | CDKN1B; CDKN4; KIP1; MEN1B; MEN4; P27KIP1; cyclin-dependent kinase inhibitor 1B; cyclin dependent kinase inhibitor 1B |
| Gene ID | 1027 |
| Uniprot ID | P46527 |
| GeneCards | CDKN1B |
| HGNC | 1785 |
| OMIM | 600778 |
| Involvement in Disease | Diseases associated with CDKN1B include Multiple Endocrine Neoplasia, Type Iv and Inherited Cancer-Predisposing Syndrome. |
| Function | Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. |
For Research Use Only.
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