Mouse SMAC antibody pair set for sandwich ELISA useCapture Antibody: Monoclonal IgGDetection Antibody: Unconjugated-Rabbit Monoclonal IgG
Specific Inquiry(Note: Please select the requirements to fit you project. If you have any other requirements, please specify. If no specific requirements, you don't need to select any button.)
Antigen Detail | |
|---|---|
| Target | SMAC |
| Description | Diablo homolog (DIABLO) is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12.[4][5][6] DIABLO is also referred to as second mitochondria-derived activator of caspases or SMAC. This protein binds inhibitor of apoptosis proteins (IAPs), thus freeing caspases to activate apoptosis.[6][7] Due to its proapoptotic function, SMAC is implicated in a broad spectrum of tumors, and small molecule SMAC mimetics have been developed to enhance current cancer treatments. |
| Species Reactivity | Mouse |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone | IV01673 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rabbit |
| Conjugation | Unconjugated |
| Clone | RD10475 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, BSA and Azide free |
General Product Property | |
|---|---|
| Product Overview | Mouse SMAC antibody pair set for sandwich ELISA use Capture Antibody: Monoclonal IgG Detection Antibody: Unconjugated-Rabbit Monoclonal IgG |
| Specificity | Both the capture and the detection antibodies in this set react with Mouse SMAC . |
| Research Area | Cardiovascular; Signal Transduction; Cancer |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | DIABLO |
| Full Name | Diablo IAP-Binding Mitochondrial Protein |
| Background | Diablo homolog (DIABLO) is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12.[4][5][6] DIABLO is also referred to as second mitochondria-derived activator of caspases or SMAC. This protein binds inhibitor of apoptosis proteins (IAPs), thus freeing caspases to activate apoptosis.[6][7] Due to its proapoptotic function, SMAC is implicated in a broad spectrum of tumors, and small molecule SMAC mimetics have been developed to enhance current cancer treatments. |
| Alternative Names | DIABLO; DFNA64; SMAC; Diablo; Diablo homolog; diablo IAP-binding mitochondrial protein |
| Gene ID | 56616 |
| Uniprot ID | Q9NR28 |
| GeneCards | DIABLO |
| HGNC | 21528 |
| Ensembl | ENSG00000184047 |
| OMIM | 605219 |
| Involvement in Disease | Diseases associated with DIABLO include Deafness, Autosomal Dominant 64 and Autosomal Dominant Non-Syndromic Sensorineural Deafness Type Dfna. |
| Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. |
For Research Use Only.
Related products Application
