Exosomes are small membrane vesicles enveloping various RNAs, proteins, lipids, and bioactive metabolites. The circRNAs in exosomes have been widely indicated to be closely involved in tumor cell proliferation and tumor invasion. As a professional service provider in the field of exosome research, Creative Biolabs devotes to offering high-level and cost-efficient exosomal circRNA sequencing services for further tumor biomarker identification.

A Brief Introduction to Exosomal circRNA

First discovered in 1983 in sheep reticulocytes, exosomes were nano-vesicles with 30-150 nm in diameter secreted by multiple cell types under both normal and pathological conditions. As the deepening of research, exosomes are not only extracellular nanovesicles, but also are specifically secreted phospholipid bilayer vesicles carrying broad host cell-derived cargo, including functional proteins, lipids, nucleic acids RNAs, and others.

Circular RNA (circRNA), a kind of non-coding RNA, is a single-stranded RNA with a special circular covalently bonded structure, in which the downstream 3’ splice donor of pre-mRNA is covalently bound to upstream 5’ splice acceptor in reverse order. The closed structure of circRNA lacking a 5’ cap and 3’ tail makes it more stable and has a higher resistance to RNase degradation. The expression and abundance of circRNAs vary in a tissue- or cell-specific manner. Large numbers of circRNAs have been identified in human transcriptomes, especially in serum exosomes. RNA sequencing technology has detected that circRNAs were enriched in exosomes in comparison to the parental cells. Furthermore, exosomal circRNAs involve in a range of biological functions, including but not limited to:

• Act as miRNA sponges, regulating the miRNA target gene expression.
• Bind to RNA binding proteins.
• Promote or inhibit cell proliferation.
• Contribute to tumor cell survival, invasion, and metastasis.

Fig.1 Biological functions of exosomal circRNAs. (Seimiya, 2020)

Exosomal circRNA Sequencing

Studies have shown that exosomal circRNAs play crucial roles in tumor cell proliferation. And the important roles of exosomes in intercellular communication may make a great contribution to tumor invasion and metastasis. Moreover, the dysregulated circRNAs in the exosome may also alter the tumor microenvironment and associate with chemotherapy drug resistance. These important actions in tumor development, together with their stable circular structure, abundance, and tissue specificity, endow exosomal circRNAs with huge potential as new biomarkers for the diagnosis of human tumors.

The powerful high-throughput RNA sequencing is the preferred technology for exosomal circRNA profiling and biomarker identification. The seasoned next-generation sequencing of exosomal circRNA from specific tissues or fluids can provide a series of important information, such as:

1. Profiling known or target circRNAs under specific or diseased conditions.
2. Identifying novel circRNAs.
3. Identifying circRNAs biomarkers for disease classification and diagnostics.
4. Exploring the circRNAs networks and function mechanisms.

Fig.2 Exosomal circRNAs may serve as a potential biomarker for cancer detection. (Li, 2015)

For Exosomal circRNA Sequencing Services, Chose Creative Biolabs

Equipped with seasoned experts and advanced platforms, Creative Biolabs is skilled at providing qualified exosome products and services for preclinical research. We have a one-stop and standard exosomal circRNA sequencing service process to help you gain reliable data with high reproducibility.

If you have different samples for exosomal circRNA sequencing, please feel free to contact us for detailed sample preparation requirements and quotations.

References

1. Seimiya, T.; et al. Emerging Roles of Exosomal Circular RNAs in Cancer. Frontiers in Cell and Developmental Biology. 2020, 8: 568366.
2. Li, Y.; et al. Circular RNA is enriched and stable in exosomes: a promising biomarker for cancer diagnosis. Cell Research. 2015, 25: 981-984.

• Exosomal Small RNA/miRNA Sequencing
• Exosomal lncRNA Sequencing
• Exosomal mRNA Sequencing
• Exosomal mRNA Microarray
• Exosomal miRNA Microarray
• Exosomal LncRNA Microarray
• Exosomal ceRNA Microarray
• Exosomal Whole Transcriptome Sequencing