Exosomal lncRNAs have been demonstrated to play important roles in human tumorigenesis and drug resistance, showing great potential to be developed as invasive diagnostic and prognostic markers for cancers. Based on the advanced technologies and experienced expert team, Creative Biolabs is happy to provide high-quality exosomal lncRNA sequencing services to customers all through the world.

The Importance of Exosomal lncRNA

All the physiological activities of organisms are strictly regulated by various gene-coding proteins in a cell-specific manner. However, the coding genes that can be transcribed into functional proteins make up only an extremely small part of the mammalian genome, the vast majority of which produce a large number of non-coding RNAs. Long non-coding RNA (lncRNA) is an important category of non-coding RNA with lengths over 200 nucleotides and lacking protein-coding potential. Diverse types of lncRNAs have been discovered and annotated, such as mRNA-like intergenic transcripts, long intronic ncRNAs, antisense RNAs, promoter-associated long RNAs, etc., all of which are implicated in a range of physiological processes and diseases. lncRNA serves as a critical regulator in DNA replication timing, gene transcription, post-transcriptional mRNA processing, and chromosome stability. Recent studies indicated lncRNA also is an important player in the pathogenesis of human diseases, especially in cancers.

LncRNAs widely distribute in various cell compartments and organelles, including in nucleoli, cytoplasmic mitochondria and ribosomes, and exosomes, among which exosomes have relatively enriched lncRNA in comparison with nuclear and cytoplasmic origin. And exosomes are rich lncRNA reservoirs. Increasing studies indicated that exosomal lncRNAs are promising and useful biomarkers in chronic inflammations and cancers, due to their higher level, RNases resistance, and tissue-specific expression.

Fig.1 Classification of lncRNA functions. (Salehi, 2017)

Exosomal lncRNA Sequencing

Dysregulation of lncRNAs exhibits adverse impacts on cellular functions. And the fact that aberrant expression of lncRNAs plays critical roles in tumorigenesis, cancer development, metastasis, and drug resistance has been widely reported. Thus, lncRNAs, particularly tissue-specific exosomal lncRNAs, hold great potential as powerful biomarkers for invasive diagnosis and prognosis of various cancer, such as glioblastoma, breast cancer, liver cancer, and so forth.

In recent years, gene high-throughput sequencing has emerged as a powerful technology for DNA and RNA expression profiling and analysis. Currently, exosomes with enough quantities can be conveniently extracted from biological samples through different approaches. Then, lncRNAs will be rapidly and precisely profiled and analyzed by RNA isolation, library preparation, high-throughput sequencing, and bioinformatics analysis. And then, the difference and potential lncRNA markers could be identified by comparing and analyzing the exosomal lncRNA from the control group and individuals with cancers.

Fig.2 Exosomal lncRNAs promote metastasis, drug resistance, and angiogenesis in cancer cells. (Yousefi, 2020)

Exosomal lncRNA Sequencing Services Provided by Creative Biolabs

With the strong strength of technology platform and abundant experience in the exosome field, Creative Biolabs has established a sound service system to provide better and comprehensive products and services for exosome research. Here, we have absolute advantages in offering qualified exosomal lncRNA sequencing services:

• Highly advanced and special SuPrecision™ Sequencing Platform.
• Highly qualified service as the premise.
• Highly experienced scientists and staff specialized in next-generation sequencing.
• Highly customized service according to different research demands.

Welcome inquiry, our experienced technicians will give you the most detailed answers to your questions.

References

1. Salehi, S.; et al. State of the art technologies to explore long non-coding RNAs in cancer. Journal of cellular and molecular medicine. 2017, 21(12): 3120-3140.
2. Yousefi, H.; et al. Long noncoding RNAs and exosomal lncRNAs: classification, and mechanisms in breast cancer metastasis and drug resistance. Oncogene. 2020, 39: 953-974.

• Exosomal Small RNA/miRNA Sequencing
• Exosomal circRNA Sequencing
• Exosomal mRNA Sequencing
• Exosomal mRNA Microarray
• Exosomal miRNA Microarray
• Exosomal LncRNA Microarray
• Exosomal ceRNA Microarray
• Exosomal Whole Transcriptome Sequencing