Exosomal miRNA Microarray

Exosomal miRNA has been confirmed as a nonnegligible player in the initiation and progression of tumors and cancers. To accelerate the exploration of the role of exosomal miRNA in cancers and the identification of exosomal miRNA biomarkers, Creative Biolabs, an expert institution and reliable service supplier, offers optimized exosomal miRNA microarray services to global customers.

Small Exosomal miRNA, Big Roles

Exosomes are lipid bilayer vesicles having 30-100 nm in diameter naturally released from most cell types by cell membrane inward budding. Consequently, exosome carries many parental cell components, such as functional proteins, lipids, and cellular metabolites. Additionally, abundant and various nucleic acids have also been identified in exosomes, including both coding and non-coding RNAs.

Among identified exosomal RNAs, microRNAs (miRNAs) are those small non-coding RNAs with about 22 nucleotides. The non-coding miRNAs are important regulators in post-transcriptional gene silencing through binding to complementary binding to the site of the 3’-untranslated region or open reading frame of mRNA. These exosomal miRNAs could be transferred between cells with exosome vesicles, and thus negatively regulating the biofunctions of recipient cells. The importance of miRNA is mainly embodied in its regulation of tumor cell activities, such as cell proliferation, survival, migration, invasion, and so forth. The exosomal miRNAs could serve as both suppressors and promoters for tumor cells, even also have an indirect influence on tumor microenvironment and immune system, emerging as promising biomarkers for minimally invasive diagnosis of tumors.

Fig.1 Mechanism of exosomal miRNA secretion. (Guo, 2020)

Exosomal miRNA Microarray

Currently, exosomal miRNAs are mainly profiled and analyzed by RNA sequencing and microarray technologies. Exosomal miRNA sequencing is directly sequencing the exosomal miRNA-based cDNA library by advanced next-generation sequencers, while, exosomal miRNA microarray captures and detects the target exosomal cDNA sequences through predesigned labeled nucleic acid probes on the chip. In essence, exosomal miRNA sequencing is to directly profile unknown miRNAs, and exosomal miRNA microarray is indirect profiling unknown miRNAs by known nucleic acid probes. Compared with exosomal miRNA sequencing, exosomal miRNA microarray has features (both advantages and limitations) of:

Easy to perform and relatively lower cost.
A large number of samples can be analyzed simultaneously.
No need for extensive and complex bioinformatic analysis.
Characterize the miRNA levels based on visualized fluorescence intensity.
Predesigned probes are required.
Not applicable to the identification of isoforms and novel genes.

Fig.2 Differential miRNA expression in urine exosomes determined by miRNA microarray. (Gildea, 2013)

Customized Exosomal miRNA Microarray Services at Creative Biolabs

At Creative Biolabs, we have advanced technology platforms, professional scientists, and highly experienced staff to provide you with a full range of standard and tailored exosome services. Here, our technicians offer optimized exosomal miRNA microarray tests, including probe predesign, exosomal miRNA isolation, and microarray analysis. Of course, exosomal miRNA sequencing services are also our specialty. For exosomal miRNA research, please choose the corresponding service according to your demands and objectives.

If there is no idea for the exosomal miRNA service you chose, please do not hesitate to contact us for technical support and a tailored solution.

References

Guo, Q.R.; et al. The Role of Exosomal microRNA in Cancer Drug Resistance. Frontiers in Oncology. 2020, 10: 472.
Gildea, J.J; et al. Urinary exosome miRNome analysis and its applications to salt sensitivity of blood pressure. Clinical Biochemistry. 2013, 46(12): 1131-1134.

Plant Exosome Isolation