Exosomal Whole Transcriptome Sequencing
Overview
The research object of whole transcriptome sequencing is all RNA that can be transcribed by a specific cell in a certain functional state, mainly mRNA and non-coding RNA such as lncRNA and circRNA. Exosomes carry important information such as a variety of proteins, lipids, short-chain RNA, and long-chain RNA from the parent cell. Whole transcriptome sequencing and analysis of long-chain RNA in exosomes can screen the specific biomarkers carried by exosomes and have important significance for the in-depth understanding of the internal mechanisms of diseases or different developmental stages. Creative Biolabs has complete exosomes whole transcriptome sequencing system, which fully combines patented micro nucleic acid library construction technology, NGS technology, and a new biological information sequence comparison algorithm. RNAs such as circRNA and circRNA are fully sequenced to obtain comprehensive exosomal transcriptome information.
Services at Creative Biolabs
Creative Biolabs provides a comprehensive exosome services platform including exosome isolation, characterization, library preparation, NGS sequencing, and data analysis. Customers only need to provide the original samples and we will help you complete the following steps.
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Comprehensive detection type of RNA
Conventional transcriptome sequencing uses the Oligo d(T) method to purify RNA molecules with Poly(A) tails. However, many popular RNA molecules, such as circular RNA and some lncRNA, do not have a Poly(A) tail, so they will be lost during processing. Creative Biolabs has complete rRNA removal kits for different species, which can fully retain the type of RNA information.
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Suitable for degradation samples
Creative Biolabs has unique kits suitable for degrading samples, which can retain the RNA information in the degraded samples to a greater extent. Especially suitable for transcriptome detection of clinical samples.
Many gene loci will generate antisense RNA with the opposite transcription direction and important regulatory functions. Conventional library building methods cannot distinguish these antisense RNAs, while Creative Biolabs uses a chain-specific library building method that can accurately detect antisense RNAs, thereby providing more comprehensive transcriptome information.
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Professional biological information analysis
Creative Biolabs has a strong bioinformatics team and is capable to meet customers' various in-depth data analysis requirements.
Fig.1 Various types of analysis.
Creative Biolabs provides comprehensive one-stop exosome research services to help accelerate our customers' researches. If you are looking for comprehensive exosome transcriptome sequencing results, or you have any puzzles during your exosomal research, please don't hesitate to contact us for professional solutions from the Ph.D. scientist team.
For Research Use Only. Cannot be used by patients.
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