Exosomal mRNA Sequencing

The exosome is an ideal research target for biomarker identification of various diseases, particularly tumors and cancers. As a reputational service provider in the field of exosome research, Creative Biolabs devotes to offering high-level and cost-efficient exosomal mRNA sequencing services for customers all over the world.

Background of Exosomal mRNA Sequencing

In the mid-20^th century, scientists have discovered a heterogeneous group of ‘pro-coagulant platelet-derived particles’ by ultracentrifugation and electron microscopy. Since then, increasing studies have been published focusing on these particles, which later were known as extracellular vesicles. Extracellular vesicles are cell-derived particles with a lipid bilayer membrane that are secreted by almost all types of cells. Based on the size and biogenesis, extracellular vesicles are generally classified into apoptotic bodies, microvesicles, and exosomes.

Exosomes, also known as intraluminal vesicles, are the smallest type of extracellular vesicles with a 30-150 nm diameter. Exosomes are produced by most eukaryotic cells and can be widely detected in all biological fluids, including blood, urine, breast milk, saliva, cerebral spinal fluid, etc. Importantly, exosomes have a unique and complex composition carrying broad host cell-derived cargo. In addition to a large number of identified proteins and lipids, enriched nucleic acids (i.e., mRNAs and miRNAs) have been profiled. Moreover, the vesicular structure of the exosome protects these RNAs from degradation and facilitates their intercellular transmission, thereby affecting the behaviors and functions of recipient cells. And, the different constituents and levels of mRNAs in exosomes closely associate with the physical and pathological conditions of originating cells.

Fig.1 Exosomes’ role in cell-cell communication and their content. (Maia, 2018)

Exosomal mRNA Sequencing

In contrast to the functional protein expression reflected by the cell transcriptome, the composition and content of the exosomal mRNA are quite different from that of the parental cell transcriptome and are closely related to intercellular communication and disease status. The characterization of exosomal mRNAs not only provides new insights into the exosomal functions, cell-cell communications, and cell pathophysiology but also contributes to the identification of exosomal mRNA biomarkers for disease diagnosis. Next-generation sequencing (NGS), or high-throughput sequencing, is a recognized and rapid technique for exosomal mRNA profiling and analysis. Creative Biolabs has launched special exosomal mRNA sequencing services added by the well-constructed and powerful technology platform. Our standard one-stop exosomal mRNA sequencing service is summarized in the following steps:

Exosome preparation, including exosome isolation and purification.
Exosome total RNA extraction, mRNA separation, and mRNA sample QC.
mRNA library preparation and library QC.
Sequencing by Illumina sequencers.
Raw data processing, bioinformatics analysis, and report delivery.

Fig.2 Exosomal mRNA sequencing service.

Exosomal mRNA Sequencing Sample Requirements

Sample Type	Requirements
Serum	5 mL
Plasma	5 mL
Cell supernatant	20 mL
Urine	100 mL
Cerebrospinal fluid	10 mL
Saliva	15 mL
Runny nose	15 mL
Exosome total RNA	≥2 μg; OD260/280≥1.8; OD260/230≥1.5

Advantages of Our Exosomal mRNA Sequencing Services

Creative Biolabs is confident in providing qualified exosome products and services for preclinical research based on seasoned experts and advanced platforms. We have a one-stop and standard exosomal mRNA sequencing service process to help you gain reliable data with high reproducibility.

Expert scientists and staff that work closely with our clients to provide satisfying service support.
Streamlined services from sample preparation to data interpretation.
Ensured comprehensive and reproducible results, and timeliness of delivery.

If you have other samples for exosomal mRNA sequencing, please feel free to contact us for detailed sample preparation requirements and quotations.