Skin Disease Model Construction Service for Exosome Functional Research

With the deepening of research, exosomes have been proven to be unique lipid vesicles that can deliver various bioactive molecules, such as proteins, drugs, and microRNA, and have functions such as participating in intercellular communication, regulating the immune system, and transporting substances. In recent years, researchers have linked exosomes to skin diseases and found that exosomes are involved in the physiological and pathological processes of the skin. In skin physiology, exosomes can regulate the secretion of cytokines in the skin microenvironment, promote angiogenesis and collagen deposition in skin defects, and regulate the proliferation and differentiation of skin fibroblasts. However, when the skin microenvironment is lesions, exosomes can promote the formation of hypertrophic scars, skin sclerosis and dermatitis, and other skin diseases. Excitingly, more and more studies suggest that natural or engineered exosomes can effectively penetrate lesions to treat these skin diseases. Establishing a suitable animal model of skin diseases is a favorable bridge to studying the efficacy evaluation of exosome drugs. Creative Biolabs has been concerned with skin care-related research and can provide the most comprehensive one-stop service for exosome research, including exosome extraction, exosome identification, exosome engineering, exosome labeling, and in vivo and in vitro verification of exosomes, to help customers further transform their achievements.

Fig.1 Study design for the induction of atopic dermatitis (AD) in the animal model using 2,4-dinitrochlorobenzene (DNCB). (Sung, 2021)

Creative Biolabs Skin Disease Model Library for Exosome Functional Research

We can provide including but not limited to the following skin disease animal models for exosome functional research.

Skin Disease Animal Models	Inducer	Induction Mechanism	Applicable Animals	Model Features
AD animal models	DNCB induction	DNCB can be covalently combined with the soluble part of the epithelial protein to form a complete antigen, which can stimulate the body to produce sensitized lymphocytes. When the sensitized lymphocytes contact the DNCB re-entering the body, it will trigger AD with Th1 cell immunity as the main factor.	Mouse	Continuous and repeated exposure to DNCB can induce Th2-type skin inflammation in mice similar to human AD.
AD animal models	Ovalbumin (OVA) induction	After repeated administration of OVA, in the local skin, acanthosis, severe epidermal spongy edema, dermal vasodilation and congestion, collagen fiber hyperplasia, and increased expression of pro-inflammatory factors will appear. This eventually triggers dermatitis dominated by Th2-type cellular immunity.	Mouse	OVA can induce Th2-type skin inflammation in mice similar to human AD.
Skin-aging animal models	D-galactose induction	Excessive administration of D-galactose can cause metabolic disorders and changes in oxidase activity. This leads to more oxidative production, causing cellular damage and reduced body function.	Mouse	The modeling is simple and the model lasts for a long time.
Skin-aging animal models	Prolonged ultraviolet exposure	Long-term ultraviolet exposure changes the degradation of collagenase and proteolytic enzymes, causing damage to collagen tissue and aging-related changes in skin texture.	Mouse, Rat	The modeling is simple and the model lasts for a long time.
Skin-scarring animal models	Ear partial incision	Excision of localized skin results in hyperkeratinization of keratinocytes, which mimics the local appearance of cutaneous scarring.	Rabbit	The modeling success rate is high. This model can better reflect the pathological changes of human hypertrophic scar formation.
Skin-scarring animal models	Full-thickness skin defect on the back	After the full-thickness skin defect is healed, there will be an excessive proliferation of fibroblasts, melanin deposition, and finally the formation of skin scars.	Rat	The model has a large scar area, which can better simulate the formation of large-area scars in humans.

Animal experiments are an important part of pharmaceutical research and development. Appropriate animal models can help researchers to more effectively understand the development of human diseases and evaluate candidate drugs. Creative Biolabs has been focusing on the development of exosome drugs in the field of skincare and skin diseases. Our animal experiment team with many years of working experience can strictly control every link for animal modeling and animal experiments. Please contact us to put forward your ideas about animal experiments, and we will recommend the most suitable animal models for skin diseases and formulate the most rigorous in vivo efficacy evaluation plan for you.

Reference

Sung, M.; Sung, SE.; et al. Serum-Derived Neuronal Exosomal microRNAs as Stress-Related Biomarkers in an Atopic Dermatitis Model. Biomedicines. 2021. 9(12).

Plant Exosome Isolation