Eye Disease Model Construction Service for Exosome Functional Research

Exosomes are a special type of extracellular vesicles that can be derived from mesenchymal stem cells, endothelial cells, hematopoietic cells, and tumor cells. Exosomes can promote the repair and regeneration of damaged tissues or cells. Through communication with cells, exosomes can promote cell migration and proliferation, reduce oxidative stress, and transfer specific biologically active substances to target cells or damage sites. With the aging population and changes in lifestyle, the incidence of eye diseases is gradually increasing, and its treatment has become a research hotspot. Due to the advantages of low immunogenicity and maintaining biochemical activity during storage, the mechanism of action of exosomes in the treatment of eye diseases has become a research hotspot. Therefore, exosomes have broad prospects for development as a cell-free substitute for stem cell therapy. The ability of exosomes to repair and regenerate has been validated in eye disease models. Creative Biolabs is actively concerned about human eye health and the research progress of exosomes in the treatment of eye diseases. We have established the most professional one-stop platform for exosome research, which can provide global customers with standardized eye disease animal model construction services for in vivo function research of exosomes.

Fig.1 CLU seals the ocular surface barrier damaged by BAC. (Chintala, 2023)

Creative Biolabs Eye Disease Model Library for Exosome Functional Research

We can provide including but not limited to the following eye disease animal models for exosome functional research.

Eye Disease Animal Models	Inducer	Induction Mechanism	Applicable Animals	Model Features
Dry eye disease (DED) animal models	Benzalkonium chloride (BAC) induction	BAC is a commonly used preservative in clinical eye drops, and it is also a common factor that causes DED clinically. The toxic effect of BAC can cause punctate peeling of the corneal epithelium and decrease the stability of the tear film.	Mouse, Rat	This method can cause tear film stability impairment, ocular surface inflammation, corneal epithelial cell apoptosis and goblet cell loss, squamous metaplasia, etc. These pathological changes are similar to human DED.
Dry eye disease (DED) animal models	Combined induction of scopolamine and evaporative hyperactivity	Scopolamine is a peripheral anticholinergic drug that competitively inhibits lacrimal gland M receptors by inhibiting parasympathetic nerve excitation and significantly reduces tear secretion. The dry environment simulated by compound fans can further lead to DED.	Mouse	This method can cause signs of DED such as a significant decrease in tear secretion, a decrease in the number of conjunctival goblet cells, and an increase in corneal fluorescence staining.
Diabetic retinopathy (DR) animal models	Streptozotocin (STZ) induction	STZ has a selective destruction effect on islet β cells. STZ-induced chronic progressive diabetes can lead to retinal microvascular leakage and occlusion, resulting in DR.	Rat	The modeling method is simple and economical, with good repeatability and a high modeling rate, and the induced retinal pathological changes are close to human DR.
Macular degeneration animal models	Sodium iodate induction	Sodium iodate is an antimetabolite that specifically induces degeneration of the retinal pigment epithelium, leading to macular degeneration.	Mouse, Rat	This modeling method can well simulate the pathogenesis of age-related macular degeneration.

At present, the development of therapeutic drugs for eye diseases is in full swing. Creative Biolabs has an integrated platform for eye disease model construction and detection. We can provide a full range of services on exosome research, including exosome extraction, exosome identification, exosome engineering, exosome labeling, and in vivo and in vitro verification of exosomes, to evaluate the in vivo efficacy of candidate exosome drugs for customers. If you are urgently looking for the best eye disease animal model to validate your innovative exosomes, please contact us. Our professional team will tailor the most suitable in vivo experiment program for you.

Reference

Chintala, SK.; Pan, J.; et al. Recombinant human clusterin seals damage to the ocular surface barrier in a mouse model of ophthalmic preservative-induced epitheliopathy. International Journal of Molecular Sciences. 2023. 24(2).

Plant Exosome Isolation