Particulate matter (PM), especially particulates with diameters of less than 2.5 mm, can penetrate the alveolar region and increase the risk of respiratory diseases. The proven adverse health effects of PM have stimulated various research efforts to develop methods for its detection. With years of research and development experience in phage display technology, Creative Biolabs has a group of scientists who are proficient in identifying binding-peptide with high specificity and affinity to particulate matter. We can provide the best service of identification of PM binding peptide at the most competitive cost.
The Introduction of PM
PM is a complex mixture of elemental and organic carbon, inorganic compounds such as sulfates and nitrates, as well as metal compounds. Both natural and manmade sources contribute to airborne PM. Typical detection methods include dynamic light scattering, gas chromatography-mass spectrometry, X-ray ﬂuorescence and instrumental neutron activation. Although these methods are comprehensive, these methods suffer from the drawbacks of long processing time, decreased accuracy and the use of highly complex and expensive equipment. This has stimulated efforts to develop detection methods so that counter measures can be taken. Biomolecules are one of the solutions to the complex issues present in the aforementioned system. Among various biomolecules, peptides have relatively simple structures, making them easier to synthesize and more stable to environmental ﬂuctuations. Furthermore, peptides can bind not only to biomolecules but also metal particulates. Researchers have found peptides capable of binding to metal particulates such as Ag, Au, and ZnO, making them promising candidates for PM detection.
Creative Biolabs has established the high-throughput phage display platform for phage display library screening against diverse targets. Our scientists have developed a new strategy for the identification of PM binding peptide. The process consists (1) coating of the PM, (2) incubation with premade phage display random peptide library, (3) washing the unbound peptides and bound peptides with low affinity, (4) elution and amplification of target binding peptide. After several rounds of biopanning, PM binding peptide could be selected and then binding characteristics will be further confirmed.
Creative Biolabs has rich experience in phage display library screening against different PMs. We are confident in providing top-quality service to facilitate our clients’ related project development. Please feel free to inquire us for more information.