How is the cDNA prepared?

We prepare cDNA by reverse-transcribing 2 μg of total RNA using a commercial cDNA Reverse Transcription Kit.

How many reactions can be performed with the cDNA?

1 μL of cDNA is sufficient for one PCR reaction.

Human Primary Lymphatic Fibroblast cDNA (CAT#: DBFF-0825-HX1213)

Datasheet

Overview

Human Primary Lymphatic Fibroblast cDNA is prepared through the reverse transcription of 2 μg of total RNA. Only 1 μl of cDNA can be used for a single PCR reaction. This cDNA offers a ready-to-use and convenient option for researchers.

Product Summary

Quantity:

* For Research Use Only. Not For Clinical Use.

Product Description

Species

Human

Tissue Name

Lymph

Size

20 reactions

Concentration

Batch-dependent

Shipping

Dry ice.

Storage

Store at -20°C. Avoid repeated freezing and thawing.

Applications

Used for gene expression analysis, including RT-PCR, real-time PCR, and other molecular biology assays.

Technical Specifications

Quality Control

cDNA is assessed for purity (A260/A280 1.7-1.9) and validated in a control PCR using β-actin primers.

FAQs

How is the cDNA prepared?
We prepare cDNA by reverse-transcribing 2 μg of total RNA using a commercial cDNA Reverse Transcription Kit.
How many reactions can be performed with the cDNA?
1 μL of cDNA is sufficient for one PCR reaction.

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For Research Use Only | Not For Clinical Use