3D Biology Based Hepatotoxicity Evaluation Services

Evaluating hepatotoxicity is a critical component of the drug development process, ensuring the safety and efficacy of pharmaceutical innovations. Utilizing state-of-the-art 3D models, Creative Biolabs provides accurate and reliable assessments of the toxicity levels of various substances and enhances the predictive accuracy of early-stage experiments.

Robust, Mature, and Reliable Hepatotoxicity Evaluation

Creative Biolabs is prepared to provide various 3D in vitro strategies to assist you in coordinating essential characterization methods. You can construct a liver toxicity evaluation roadmap tailored to your objectives for comparative analysis, or leverage the advantages of a specific model to make more informed risk assessment decisions.

Hepatotoxicity Evaluation by Liver Organoid Model	The liver organoid model, assembled from multiple types of cells, enhance liver phenotypes and examine specific functions and readings. In general, our optimized cultivation/differentiation procedures enable us to construct a rational liver organoid system using iPSCs, and introduce specific injuries into the model through gene editing.
Drug-induced Liver Injury (DILI) Evaluation by 3D Microtissue Model	By co-cultivating primary human liver cells and hepatic endothelial cells, we have created a highly specific 3D liver microtissue model that provides additional predictive sensitivity for your DILI (Drug-Induced Liver Injury) testing while ensuring differentiated cell phenotypes and functions.
Bile Acid-Dependent Hepatotoxicity Evaluation by Human Hepatocytes	Primary human liver cells cultured in a bile acid mixture can predict DILI and offer high-quality and reproducible results for drug transporter protein assays. Our stable operational procedures enable us to provide you with rapid turnover and accurate liver toxicity risk assessments within one week.
Hepatotoxicity Evaluation by iPSC-derived Hepatocyte Spheroids	iPSC-derived hepatocyte spheroids provide rich and customizable cell sources for liver toxicity research. We implement high-throughput confocal imaging acquisition using a wash-free fluorescent staining protocol, and can also provide toxicity evaluations for a wider range of phenotypic parameters.

2D Model or 3D Hepatotoxicity Evaluation?

2D Liver Cell Culture	Face the challenge of early dedifferentiation in culture
	Loss of relevant gene expression, epithelial polarity, specific protein synthesis, and crucial enzyme activity
	Primary liver cells gradually lose their hepatic functions within a short period
3D Evaluation	Robust urea production, maintain the activity of phase i and ii metabolic enzymes
	Sustained albumin secretion over extended periods ranging from days
	Preserve tricarboxylic acid cycle, glycolysis, and mitochondrial functionality
	Exhibit a high level of protein expression relevant to drug absorption, distribution, metabolism, and excretion

Customizable Services for Hepatotoxicity Evaluation

Creative Biolabs has the capability to optimize the accuracy of your drug candidate's evaluation regarding liver toxicity liability. We offer optional models, cell sources, and testing focuses to provide you with reliable, predictive, and significant toxicological and pharmacological results, encompassing ATP and LDH assays, as well as additional mechanistic endpoint analysis.

Hepatotoxicity. (Serras, 2021) Fig 1. Hepatotoxicity.¹

As a preeminent biotechnology enterprise, Creative Biolabs provides models that faithfully replicate physiological drug responses, exhibiting biomimicry, thus rendering them suitable for various toxicity screening methodologies. Contact us today and let our 3D biology-based expertise take your research to new heights. Our dedicated team is here to provide tailored solutions and ensure the safety of your innovations.

Reference

Serras, S.A.; et al. A critical perspective on 3D liver models for drug metabolism and toxicology studies. Frontiers in Cell and Developmental Biology. 2021, 10: 626805.

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3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

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