How is the miRNA prepared?

The miRNA is purified through organic extraction and enriched by binding RNA to glass-fiber filters. It is then eluted and stored in nuclease-free water.

From which passage are the miRNAs prepared from primary cells?

The specific cell passage can be provided upon request. Generally, we obtain the miRNA from cells with no more than passage 3.

Human Primary Trabecular Meshwork Cell MiRNA (CAT#: DBFF-0825-HX1611)

Datasheet

Overview

Human Primary Trabecular Meshwork Cell MiRNA is obtained from primary cells at a lower passage through an organic extraction process, followed by RNA enrichment using glass-fiber filters. The purified miRNA is then eluted and stored in nuclease-free water. This miRNA product provides a practical and ready-to-use alternative for researchers who are looking for tissues to isolate cells and extract miRNA.

Product Summary

Quantity:

* For Research Use Only. Not For Clinical Use.

Product Description

Species

Human

Tissue Name

Trabecular meshwork

Size

1 ug;2 ug;5 ug

Concentration

Batch-dependent

Shipping

Dry ice.

Storage

Store at -80°C upon arrival. Avoid repeated freeze/thaw cycles.

Applications

PCR, qPCR, genotyping, sequencing, cloning, and epigenetic studies such as methylation analysis.

Technical Specifications

Quality Control

MiRNA quality is verified by an A260/A280 ratio of 1.8-2.1.

FAQs

How is the miRNA prepared?
The miRNA is purified through organic extraction and enriched by binding RNA to glass-fiber filters. It is then eluted and stored in nuclease-free water.
From which passage are the miRNAs prepared from primary cells?
The specific cell passage can be provided upon request. Generally, we obtain the miRNA from cells with no more than passage 3.

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For Research Use Only | Not For Clinical Use