This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (CAT-CBh-Sec/SPII-CD79α-CD8-HA) and CRISPR-Cas (Cas12b (C2c1)-RNARuvC, HNH-NNNNGATT-attP) systems.
| Clone | BGS7-S033 |
| Host | Mouse |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | Luc |
| Promoter | mPGK |
| Signal peptides | Sec/SPII |
| Signaling domains | CD79α |
| TM domains | CD8 |
| Detection tags | HA |
| Linkers | Flexible Linker |
| Co-Receptors | Siglec-G |
| Vector Type | MSCV |
| Vector Name | BGpCDH6 |
| Class | 2 single-Cas |
| Type | II |
| Subtype | A, B, C, D, E |
| Effector | Cas12b (C2c1) |
| DNA Type | RNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | No |
| PAM/PFS | NNNNGATT |
| Insertion | attP |
| Construction | Luc-mPGK-CD79α-Cas12b (C2c1)-RNA-attP |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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