This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-MNDU3-Sec/SPIII-CD79α-CD28-HA) and CRISPR-Cas (Cas12a (Cpf1)-RNA-RuvC, HNH-NGG-Csn) systems.
| Clone | TIG10 |
| Host | Human |
| Targeting Cell Type | Raji-Luc2 |
| Reporter gene | CAT |
| Promoter | EF1A |
| Signal peptides | Sec/SPIII |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | HA |
| Linkers | SEG |
| Co-Receptors | CD81 |
| Vector Type | AAV |
| Vector Name | BGpCDH1 |
| Class | 2 single-Cas |
| Type | Ⅴ |
| Subtype | A, B, C, D, E, F |
| Effector | Cas12a (Cpf1) |
| DNA Type | RNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | No |
| PAM/PFS | NGG |
| Insertion | Csn |
| Construction | CAT-EF1A-CD79α-Cas12a (Cpf1)-RNA-Csn |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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