This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-CMV+intron-Sec/SPI-CD79α-CD28-CBP) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | 22RUA6 |
Host | Mouse |
Targeting Cell Type | Ramos (RA 1) |
Reporter gene | GUS |
Promoter | CMV+intron |
Signal peptides | Sec/SPI |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | CBP |
Linkers | Flexible Linker |
Co-Receptors | CD72 |
Vector Type | AAV |
Vector Name | BGpCDH1 |
Class | 1 multi-Cas |
Type | VI |
Subtype | A, B, C, D |
Effector | Cas12a (Cpf1) |
DNA Type | dsDNA |
Nuclease domains | Unknown |
TracrRNA requirement | YES |
PAM/PFS | 5' AT-rich PAM |
Insertion | ROSA26 |
Construction | GUS-CMV+intron-CD79α-Cas12a (Cpf1)-dsDNA-ROSA26 |
Target | RXFP1 |
Alternative Names | RXFP1; LGR7; RXFPR1; Relaxin/insulin-like family peptide receptor 1; relaxin/insulin like family peptide receptor 1; relaxin family peptide receptor 1 |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.