RXFP1 mAb (22RUA6) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR7)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-CMV+intron-Sec/SPI-CD79α-CD28-CBP) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone 22RUA6
Host Mouse
Targeting Cell Type Ramos (RA 1)
Reporter gene GUS
Promoter CMV+intron
Signal peptides Sec/SPI
Signaling domains CD79α
TM domains CD28
Detection tags CBP
Linkers Flexible Linker
Co-Receptors CD72
Vector Type AAV
Vector Name BGpCDH1
Class 1 multi-Cas
Type VI
Subtype A, B, C, D
Effector Cas12a (Cpf1)
DNA Type dsDNA
Nuclease domains Unknown
TracrRNA requirement YES
PAM/PFS 5' AT-rich PAM
Insertion ROSA26
Construction GUS-CMV+intron-CD79α-Cas12a (Cpf1)-dsDNA-ROSA26

Target Information

Target RXFP1
Alternative Names RXFP1; LGR7; RXFPR1; Relaxin/insulin-like family peptide receptor 1; relaxin/insulin like family peptide receptor 1; relaxin family peptide receptor 1

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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