Immunohistochemistry (IHC) is an integral technique for tissue-based diagnostics and biomarker detection that is broadly utilized worldwide. It is mostly applied in conjunction with morphologic evaluation as a diagnostic tool. Focusing on the development of in vitro diagnostic (IVD) strategies over years, Creative Biolabs has established our technology platform and provides high-quality IHC analysis services to global researchers.

Introduction of IHC

During the past three decades, IHC has evolved from a highly specialized testing modality available in a few research centers to a diagnostic mainstay performed by automated laboratories in community hospitals. This powerful adjunctive test has markedly increased diagnostic accuracy in surgical pathology by supplementing routine morphological data with molecular clues about the lineage of cells and tissues. Although in many instances IHC serves to confirm diagnostic impressions based on morphology, it may be a critical factor in difficult clinical scenarios such as the diagnosis of spindle cell and soft tissue neoplasms or poorly differentiated malignancies, and occasionally it can identify an occult primary tumor that has spawned metastatic disease.

Workflow of immunohistochemistry.Fig.1 Workflow of immunohistochemistry.
(Eagle, 2008)

Examples of IHC Application for Disease Diagnostics

  • Parathyroid tissue and tumors

IHC can be useful in the diagnosis and classification of parathyroid tissue and tumors. In difficult cases, immunohistochemical studies can be used in distinguishing parathyroid from other tissues and tumors occurring in the neck such as thyroid folliculogenic and medullary tumors as well as unusual tumors occurring in the neck.

  • Dermatopathology

IHC is a commonly employed adjunctive tool in diagnostic dermatopathology. IHC involves the use of simple immunologic methods to identify and localize characteristics of cells not visible with routine histologic methods. The role of IHC in diagnostic dermatopathology is usually to attempt to classify the tissue type or cell of origin. The most commonly used epithelial markers include cytokeratins, epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), and gross cystic disease fluid protein (GCDFP). Markers of epithelial differentiation are especially useful in determining the cell of origin of an undifferentiated neoplasm.

  • Non-neoplastic disease

IHC can be used to demonstrate the presence of microorganisms in tissue sections, particularly viruses such as herpes simplex virus in the cornea and herpes simplex virus or other herpesviruses like cytomegalovirus or varicella-zoster virus in necrotizing retinitis. IHC is also used to confirm the presence of carcinogenic strains of human papillomavirus (HPV) in ocular surface squamous lesions (e.g. HPV 16 or 18), particularly when histologic features such as koilocytosis suggest a viral etiology. Demonstration of nuclear staining for human herpesvirus is an important diagnostic criterion for Kaposi sarcoma.

Services at Creative Biolabs

Immunohistochemistry Analysis

With years of experience focusing on the development of IVD, Creative Biolabs has accumulated extensive expertise and mature technology. We are confident in offering customer-satisfied IHC analysis services to global customers. Our comprehensive technology platform, excellent scientific staff, and rich experience are the powerful guarantee of our services.

  • Hundreds of successful precedents
  • Great industrial reputation
  • Strong experimental foundations with mature technicians

As an industry-leading biotechnology company, Creative Biolabs has never stopped making progress. We aspire to satisfy every client and become the best scientific service provider. If you are interested in IHC analysis services or any other IVD development services, please feel free to contact us for more information.

Reference

  1. Eagle, R. C., Jr. Immunohistochemistry in diagnostic ophthalmic pathology: A review. Clin Exp Ophthalmol. 2008, 36(7): 675-88.

For Research Use Only.


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