Creative Biolabs is a dedicated IVD provider that strives to offer our global customers the highest quality services. We have developed a sensitive platform for multiplexed protein and DNA analysis based on integrating Microfluidics to achieve your specific project demands.

Microfluidics, featuring microfabricated structures, is a technology for manipulating fluids at the micrometer scale. The small dimension and flexibility of Microfluidic systems are ideal for multiplexed protein and DNA analysis and show great potential in research and diagnosis. Researchers have recently reported highly-integrated and parallelized Microfluidic-based tests, allowing the simultaneous detection and analysis of both protein and DNA samples in a single test. The target DNA sequence encodes the outer membrane protein P6 of Haemophilus influenzae, and the pro-inflammatory mediators IL-6 and IL-8 are selected as target proteins. In this system, rolling circle amplification (RCA) is used as a signal amplification method. A two-layer sandwich assay designs the microsphere sensors for multiplexed analysis of combined protein and DNA. With this assay, detection limits down to 10 fM and 1 pM are achieved for proteins and target DNA, respectively.

Multiplexed protein and DNA analysis.Fig.1 Multiplexed protein and DNA analysis. (Konry, 2009)

Multiplexed Protein and DNA Analysis at Creative Biolabs

Creative Biolabs has been a long-term expert in the field of IVD. We have won a good reputation for accomplishing numerous challenging projects based on our advanced Microfluidic technology and extensive experience. Our seasoned scientists have currently set up one-stop procedures that can assist your project with the highest efficiency and the best quality. We provide universal Microfluidic-based integrated approaches for our customers, which combine the rapid diagnostic assay and the efficient liquid handling capability of Microfluidics for sensitive, quantitative, and high-throughput protein and DNA analysis.

Advantages of Our Integrated Approaches

The combinations of multiplexed assays with Microfluidic systems have recently attracted increasing attention due to the high-throughput of the Microfluidic systems and the multiplexing capability to detect both genomic and proteomic information for different biological targets. Our integrated approach offers several advantages, such as all fluid handling steps can be integrated and automated. Compared with conventional systems, the benefits of this Microfluidic-based platform include low reagent consumption, better reaction efficiency, higher sensitivity, and a more comprehensive linear detection range. With our integrated Microfluidic platform, both protein and DNA could be diagnosed by a single rapid analysis, rather than testing for different markers with specific tests.

Creative Biolabs now offers an integrated approach using Microfluidic technology to develop multiplexed assays for discrimination of DNA and parallel analysis of a panel of protein markers to meet our customers' specific requirements. If you are interested in our service, please feel free to contact us for more details.

Reference

  1. Konry, T.; et al. Microsphere-based rolling circle amplification microarray for the detection of DNA and proteins in a single assay. Analytical chemistry. 2009, 81(14), pp.5777-5782.

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